Isolation and characterization of a new Methanoculleus bourgensis strain KOR-2 from the rumen of Holstein steers.

Objective: To isolate and identify new methanogens from the rumen of Holstein steers in Korea.

Methods: Representative rumen contents were obtained from three ruminally cannulated Holstein steers (793±8 kg). Pre-reduced media were used for the growth and isolation of methanogens.

Isolation and characterization of new strains KOR-3, -4, -5, and -6 from an anaerobic digester using pig slurry.

Objective: An experiment was conducted to isolate and identify new methanogens in Korea from an anaerobic digester that uses pig slurry.

Methods: An anaerobic digestate sample was collected from an anaerobic digester using pig slurry. Pre-reduced media were used for the growth and isolation of methanogens.

Isolation and Characterization of a New Methanobacterium formicicum KOR-1 from an Anaerobic Digester Using Pig Slurry.

A new methanogen was isolated from an anaerobic digester using pig slurry in South Korea. Only one strain, designated KOR-1, was characterized in detail. Cells of KOR-1 were straight or crooked rods, non-motile, 5 to 15 μm long and 0.