Genomic and biochemical investigations in the biomineralizing potential of an isolated marine ureolytic Bacillus sp. N₉.

Microbially Induced Calcium Carbonate Precipitation (MICP) plays a significant role in coastal soil stabilization and erosion prevention. In the present study, the biomineralizing potential of a newly isolated Bacillus sp. N₉ was investigated through MICP.

Integrated formic acid and deep eutectic solvent mediated sustainable synthesis of cellulose nanocrystals from shells.

The present study reports the green synthesis of cellulose nanocrystals from the shells of (SFS) cellulose. Three different methods, alkali, acid and organic acid, were screened for the maximum cellulose extraction. A maximum cellulose yield, 30.

Deep eutectic solvent assisted recovery of high molecular weight levan from an isolated BPSCM4.

The present study demonstrates the usage of deep eutectic solvent to recover microbial levan from the clarified fermented broth. The classic ethanol precipitation method for levan recovery is expensive because ethanol can be utilized as a biofuel. Production of ethanol consumes more energy and is not easily recycled.

The antibiofilm potential of a heteropolysaccharide produced and characterized from the isolated marine bacterium BPM30.

Biofilms are the significant causes of 80% of chronic infections in the oral cavity, urinary tract, biliary tube, lungs, gastrointestinal tract, and so on to the general public. Treatment of pathogenic biofilm using bacterial exopolysaccharides (EPS) is an effective and promising strategy. In the present work, a marine bacterium was isolated, studied for exopolysaccharide production, and tested for its antibiofilm activity.

Binary RuO-CuO Electrodes Outperform RuO Electrodes in Measuring the pH in Food Samples.

Glass electrodes are the only type of pH-sensitive electrodes currently used in the food industry. While widely used, they have several disadvantages, especially in the areas of brittleness and price. Ruthenium(IV) oxide (RuO) pH electrodes are a well-known alternative to conventional glass electrodes, providing improved durability and lower price.

Unwinding the mechanism of macrophage repolarization potential of sp. -derived protein protease inhibitor through Toll-like receptor 4 against experimental visceral leishmaniasis.

The sp. -derived protein protease inhibitor (PPI) has been proven to shift macrophages towards an inflammatory state and reduce infection and . The current study explored and validated the mechanistic aspects of the PPI and Toll-like receptor (TLR) interaction.

The comprehensive characterization of Prosopis juliflora pods as a potential bioenergy feedstock.

The production of renewable and sustainable biofuels using inevitable wastes is a promising alternative to the alarming depletion of fossil fuels. Significantly, the sustainable biorefinery of lignocellulosic waste, as an alternative fuel source, is a prognosticating approach to tackle many agricultural/forestry residues and offers a circular economy as well as environmental benefits. But, the heterogeneity of lignocellulosic biomass is one of the major bottlenecks in lignocellulosic biorefinery.

Oceanimonas sp. BPMS22-derived protein protease inhibitor induces anti-leishmanial immune responses through macrophage M2 to M1 repolarization.

The present study aimed to validate the potential of a novel serine protein protease inhibitor (PPI), purified from marine Oceanimonas sp. BPMS22, induced M2 to M1 repolarization of the macrophages to treat visceral leishmaniasis (VL). Peptide mass fingerprint of the purified trypsin digested PPI peptide was obtained using matrix-assisted laser desorption ionization-time of flight combined with tandem mass spectrometry (MALDI-TOF MS/MS) and the sequence was used to construct a 3D protein model by homology modelling.

Research and economic perspectives on an integrated biorefinery approach for the simultaneous production of polyhydroxyalkanoates and biohydrogen.

Alarming environmental impacts have been resulted across the globe due to the recovery and consumption of fossil fuels. The elevated global carbon footprint has paved the way to an alternative to combat the prevalent pollution. On the other hand, the fossil-based plastics produced from the byproducts of petroleum remain intact in the environment leading to pollution.

Fabrication, Potentiometric Characterization, and Application of Screen-Printed RuO pH Electrodes for Water Quality Testing.

Screen-printed sensing electrodes attract much attention for water pollution monitoring due to their small size, physical and chemical durability, and low cost. This paper presents the fabrication and broad potentiometric characterization of RuO pH sensing electrodes deposited by screen printing on alumina substrates and sintered in the 800-900 °C temperature range. All the fabricated electrodes showed close to Nernstian sensitivity, good linearity, fast response, small drift, low hysteresis, and low cross-sensitivity toward various interfering cations and anions.

Rapid Assessment of SARS-CoV-2 Transmission Risk for Fecally Contaminated River Water.

Following the outbreak of severe acute respiratory syndrome coronavirus (SARS-CoV-2), airborne water droplets have been identified as the main transmission route. Identifying and breaking all viable transmission routes are critical to stop future outbreaks, and the potential of transmission by water has been highlighted. By modifying established approaches, we provide a method for the rapid assessment of the risk of transmission posed by fecally contaminated river water and give example results for 39 countries.

Maximizing the direct recovery and stabilization of cellulolytic enzymes from Trichoderma harzanium BPGF1 fermented broth using carboxymethyl inulin nanoparticles.

The carboxymethylated inulin (CMI) nanoparticles prepared by the salt out method was demonstrated to harvest cellulolytic enzymes (Ez) directly from the clarified fermented broth of Trichoderma harzanium BPGF1. The formation of CMI nanoparticles and entrapment of Ez in CMI was confirmed by scanning electron microscopy and Fourier transform infrared spectroscopy, respectively. A factorial design was developed to maximize enzymes recovery directly from the fermented broth.

Isolation and characterization of cellulose nanocrystals from jackfruit peel.

In the present work, sustainable nanomaterials, cellulose, and spherical cellulose nanocrystals (SCNCs) were isolated from the non-edible parts of jackfruit (Artocarpus heterophyllus). Of the three different methods tested, sodium chlorite treatment produced the highest yield of cellulose, 20.08 ± 0.

Potential Anticoagulant Activity of Trypsin Inhibitor Purified from an Isolated Marine Bacterium Oceanimonas Sp. BPMS22 and its Kinetics.

Protease inhibitors control major biological protease activities to maintain physiological homeostasis. Marine bacteria isolated from oligotrophic conditions could be taxonomically distinct, metabolically unique, and offers a wide variety of biochemicals. In the present investigation, marine sediments were screened for the potential bacteria that can produce trypsin inhibitors.

Modeling of growth kinetics for an isolated marine bacterium, Oceanimonas sp. BPMS22 during the production of a trypsin inhibitor.

Protease inhibitors significantly control physiologically relevant protease activities. Protease inhibitors from marine microbial sources are unique due to their rough living environmental conditions. In the present study, a protein protease inhibitor (PI) was produced from marine Oceanimonas sp.

Microbial serine protease inhibitors and their therapeutic applications.

Serine protease inhibitors, inhibit serine proteases either partially or completely after forming complexes with their respective proteases. Protease actions are significant for many physiological pathways found in living forms and any anomalies may lead to numerous physiological complications. Each cell or organism has its own mechanism for controlling these protease actions.

Concurrent production of cellulase and xylanase from Trichoderma reesei NCIM 1186: enhancement of production by desirability-based multi-objective method.

Application of multiple response optimizations using desirability function in the production of microbial metabolites improves economy and efficiency. Concurrent production of cellulase and xylanase in Trichoderma reesei NCIM 1186 using an agricultural weed, Prosopis juliflora pods, was studied. The main aim of the study was to optimize significant medium nutrient parameters for maximization of cellulase and xylanase by multi-objective optimization strategy using biomass.

Immobilization of levan-xylanase nanohybrid on an alginate bead improves xylanase stability at wide pH and temperature.

Despite the sustainable availability, levan, a fructose based natural polysaccharide has not received significant attention in the development of enzyme immobilization technology. Herein, we prepared levan-xylanase (LXy) nanohybrid and characterized by scanning electron microscopy, particle size analyzer and zeta potential. To prevent the enzyme leakage from the nanohybrid, LXy was immobilized onto an alginate beads (NaAlg).

Formulation, characterization, in vitro and in vivo evaluation of castor oil based self-nano emulsifying levosulpiride delivery systems.

Context: Levosulpiride (LSP) is a hydrophobic benzamide derivative used in the treatment of schizophrenia. SNEDDS were extensively practiced for systemic delivery of poorly aqueous soluble drugs to achieve maximum bioavailability.

Objective: The present study was focussed on the formulation, optimisation and evaluation of LSP SNEDDS using castor oil, for enhancement of drug absorption and bioavailability.

Sequential Optimization Methods for Augmentation of Marine Enzymes Production in Solid-State Fermentation: l-Glutaminase Production a Case Study.

There is an increased l-glutaminase market worldwide due to its relevant industrial applications. Salt tolerance l-glutaminases play a vital role in the increase of flavor of different types of foods like soya sauce and tofu. This chapter is presenting the economically viable l-glutaminases production in solid-state fermentation (SSF) by Aspergillus flavus MTCC 9972 as a case study.

Synthesis of fibrinolytic active silver nanoparticle using wheat bran xylan as a reducing and stabilizing agent.

A facile synthesis of highly stable silver nanoparticles (AgNPs) was reported using a biopolymer, xylan as both a reducing and stabilizing agent. Xylan was isolated from waste biomass, wheat bran (WB) by alkaline treatment and was characterized by Fehling's test, dinitrosalicylic acid assay, FTIR, (1)H NMR and (13)C NMR. The synthesized nanoparticles were characterized by UV-Vis spectroscopy and transmission electron microscopy.

Antioxidant and anti-inflammatory levan produced from Acetobacter xylinum NCIM2526 and its statistical optimization.

Levan is a homopolymer of fructose naturally obtained from both the plants and microorganisms. Along with the general properties of a biopolymer like bio-compatibility, bio-degradability, renewability, flexibility, and eco-friendliness, levan also offers some important biomedical properties such as anti-oxidant, anti-inflammatory, anti-carcinogenic, anti-AIDS and hyperglycaemic inhibitor. In this study, we have demonstrated the microbial production of therapeutically potential levan by batch fermentation process in sucrose rich medium using Acetobacter xylinum NCIM 2526.

Review on production, characterization and applications of microbial levan.

Levan is a homopolymer of fructose naturally obtained from both plants and microorganisms. Microbial levans are more advantageous, economical and industrially feasible with numerous applications. Bacterial levans are much larger than those produced by plants with multiple branches and molecular weights ranging from 2 to 100 million Da.

Green synthesis of silver and gold nanoparticles employing levan, a biopolymer from Acetobacter xylinum NCIM 2526, as a reducing agent and capping agent.

With a vision of finding greener materials to synthesize nanoparticles, we report the production and isolation of levan, a polysaccharide with repeating units of fructose, from Acetobacter xylinum NCIM2526. The isolated levan were characterized using potassium ferricyanide reducing power assay, Fourier transform infra-red (FTIR) spectroscopy and (1)H nuclear magnetic resonance spectroscopy ((1)H NMR). To exploit levan in nanotechnology, we present a simple and greener method to synthesize silver nanoparticles (AgNPs) and gold nanoparticles (AuNPs) using biopolymer, levan as both reducing and stabilizing agents.