Publications by authors named "Upeksha Mirissa Lankage"
Sensors that can quickly measure the lipase activity from biological samples are useful in enzyme production and medical diagnostics. However, current lipase sensors have limitations such as requiring fluorescent labels, pH control of buffer vehicles, or lengthy assay preparation. We introduce a sparsely tethered triglyceride substrate anchored off of a gold electrode for the impedance sensing of real-time lipase activity.
View Article and Find Full Text PDFArticle Synopsis
- Phospholipase-A (PLA) enzymes break down specific glycerophospholipids and are important for studying venoms and diagnosing diseases like acute pancreatitis.
- Current PLA detection methods face challenges including lengthy assay preparation, reliance on fluorescent labels, and strict pH requirements.
- The new tethered bilayer lipid membrane (tBLM) impedance sensor array allows for quick, real-time detection of PLA isoforms using electrical impedance spectroscopy, showing effectiveness in identifying PLA activity from sources like the South American bullet ant venom.