Calcium Signalling in Neurological Disorders, with Insights from Miniature Fluorescence Microscopy.

Neurological disorders (NDs), such as amyotrophic lateral sclerosis (ALS), Alzheimer's disease (AD), Parkinson's disease (PD), Huntington's disease (HD), and schizophrenia, represent a complex and multifaceted health challenge that affects millions of people around the world. Growing evidence suggests that disrupted neuronal calcium signalling contributes to the pathophysiology of NDs. Additionally, calcium functions as a ubiquitous second messenger involved in diverse cellular processes, from synaptic activity to intercellular communication, making it a potential therapeutic target.

Unraveling High Altitude-Induced Thromboembolic Disorders: Polycythemia or Complex Mechanisms?

Kansara, Nikunj Kumar, Anurag Timothy, Rijesh Unnithan, and Manas Chatterjee. Unraveling high altitude-induced thromboembolic disorders: polycythemia or complex mechanisms?. 00:00-00, 2024.

Protocol for calcium imaging and analysis of hippocampal CA1 activity evoked by non-spatial stimuli.

The hippocampus has a major role in processing spatial information but has been found to encode non-spatial information from multisensory modalities in recent studies. Here, we present a protocol for recording non-spatial stimuli (visual, auditory, and a combination) that evoked calcium activity of hippocampal CA1 neuronal ensembles in C57BL/6 mice using a miniaturized fluorescence microscope. We describe steps for experimental apparatus setup, surgical procedures, software development, and neuronal population activity analysis.

A multifunctional smart field-programmable radio frequency surface.

Antennas that can operate across multiple communication standards have remained a challenge. To address these limitations, we propose a Field-Programmable Radio Frequency Surface (FPRFS), which is based on manipulating current flow on its surface to achieve desirable RF characteristics. In this work, we demonstrate that substantial enhancements in radiation efficiency can be achieved while preserving the high reconfigurability of antenna structures implemented on the FPRFS.

Hippocampal cognitive and relational map paradigms explored by multisensory encoding recording with wide-field calcium imaging.

Two major theories have been proposed to explain hippocampal function: cognitive map and the relational theories. They differ in their views on whether hippocampal neurons can process non-spatial information independently. However, the explanatory power of these theories remains unresolved.

Wearable Transmitter Coil Design for Inductive Wireless Power Transfer to Implantable Devices.

Wireless endovascular sensors and stimulators are emerging biomedical technologies for applications such as endovascular pressure monitoring, hyperthermia, and neural stimulations. Recently, coil-shaped stents have been proposed for inductive power transfer to endovascular devices using the stent as a receiver. However, less work has been done on the external transmitter components, so the maximum power transferable remains unknown.

Multicolor flow cytometry-based immunophenotyping for preclinical characterization of nanotechnology-based formulations: an insight into structure activity relationship and nanoparticle biocompatibility profiles.

Introduction: Immunophenotyping, which is the identification of immune cell subsets based on antigen expression, is an integral technique used to determine changes of cell composition and activation in various disease states or as a response to different stimuli. As nanoparticles are increasingly utilized for diagnostic and therapeutic applications, it is important to develop methodology that allows for the evaluation of their immunological impact. Therefore, the development of techniques such as immunophenotyping are desirable.

Design of an On-Chip Plasmonic Modulator Based on Hybrid Orthogonal Junctions Using Vanadium Dioxide.

We present the design of a plasmonic modulator based on hybrid orthogonal silver junctions using vanadium dioxide as the modulating material on a silicon-on-insulator. The modulator has an ultra-compact footprint of 1.8 μm × 1 μm with a 100 nm × 100 nm modulating section based on the hybrid orthogonal geometry.

Early Detection of Aphid Infestation and Insect-Plant Interaction Assessment in Wheat Using a Low-Cost Electronic Nose (E-Nose), Near-Infrared Spectroscopy and Machine Learning Modeling.

Advances in early insect detection have been reported using digital technologies through camera systems, sensor networks, and remote sensing coupled with machine learning (ML) modeling. However, up to date, there is no cost-effective system to monitor insect presence accurately and insect-plant interactions. This paper presents results on the implementation of near-infrared spectroscopy (NIR) and a low-cost electronic nose (e-nose) coupled with machine learning.

Effects of antipsychotic drugs and potassium channel modulators on spectral properties of local field potentials in mouse hippocampus and pre-frontal cortex.

Local field potentials (LFPs) recorded intracranially display a range of location-specific oscillatory spectra which have been related to cognitive processes. Although the mechanisms producing LFPs are not completely understood, it is likely that voltage-gated ion channels which produce action potentials and patterned discharges play a significant role. It is also known that antipsychotic drugs (APDs) affect LFP spectra and a direct inhibitory effect on voltage-gated potassium channels has been reported.

Scopolamine Impairs Spatial Information Recorded With "Miniscope" Calcium Imaging in Hippocampal Place Cells.

The hippocampus and associated cholinergic inputs have important roles in spatial memory in rodents. Muscarinic acetylcholine receptors (mAChRs) are involved in the communication of cholinergic signals and regulate spatial memory. They have been found to impact the memory encoding process, but the effect on memory retrieval is controversial.

The effect of smoking on cumulative damage in systemic lupus erythematosus: An incident cohort study.

Objectives: To investigate the relationship between smoking history and pack-year exposure on the rate of end-organ damage in systemic lupus erythematosus (SLE).

Methods: The SLE incident cohort included patients who met American College of Rheumatology (ACR) 1997 or SLE International Collaborating Clinics (SLICC) 2012 SLE criteria and had rheumatology encounters at a US academic institution (2008-16). The primary outcome was median time to SLICC/ACR damage index (SLICC/ACR-DI) increase or death.

Thermal denaturation of proteins in the muscle fibre and connective tissue from bovine muscles composed of type I (masseter) or type II (cutaneous trunci) fibres: DSC and FTIR microspectroscopy study.

The changes in secondary structure of proteins with heating were characterised and compared for bovine masseter (fibre type I) and cutaneous trunci (fibre type II) muscles by Differential Scanning Calorimetry (DSC) and Fourier Transform InfraRed (FTIR) microspectroscopy. Heating led to a decrease in α- helices, and an increase in aggregated strands, random coils and aromatic side chains in the muscle fibres of both muscles. In the intramuscular connective tissue (IMCT) of both muscles, a decrease in α- helix, turn and unordered structures was complemented with an increase in aggregated strands.

A Rare Case of Epileptic Seizure After-effect Resulting in Nontraumatic Spontaneous Subdural Hemorrhage.

Unlabelled: Generalized tonic-clonic seizures (GTCS) result in diverse physiological alterations that are mostly short-lived and rarely lead to immediate serious consequences. Some early serious complications reported are head trauma and aspiration. While most cases of seizures are diagnosed readily from clinical history, some cases remain indolent and present later.

Assessment of Smoke Contamination in Grapevine Berries and Taint in Wines Due to Bushfires Using a Low-Cost E-Nose and an Artificial Intelligence Approach.

Bushfires are increasing in number and intensity due to climate change. A newly developed low-cost electronic nose (e-nose) was tested on wines made from grapevines exposed to smoke in field trials. E-nose readings were obtained from wines from five experimental treatments: (i) low-density smoke exposure (LS), (ii) high-density smoke exposure (HS), (iii) high-density smoke exposure with in-canopy misting (HSM), and two controls: (iv) control (C; no smoke treatment) and (v) control with in-canopy misting (CM; no smoke treatment).

Platelet-Specific PDGFB Ablation Impairs Tumor Vessel Integrity and Promotes Metastasis.

Platelet-derived growth factor B (PDGFB) plays a crucial role in recruitment of PDGF receptor β-positive pericytes to blood vessels. The endothelium is an essential source of PDGFB in this process. Platelets constitute a major reservoir of PDGFB and are continuously activated in the tumor microenvironment, exposing tumors to the plethora of growth factors contained in platelet granules.

Clinical Significance of Monitoring Hydroxychloroquine Levels in Patients With Systemic Lupus Erythematosus: A Systematic Review and Meta-Analysis.

Objective: Despite the pivotal role that hydroxychloroquine (HCQ) plays in treating systemic lupus erythematosus (SLE), less than 50% of patients take HCQ as prescribed. Measurement of HCQ blood levels can help clinicians distinguish nonadherence versus lack of efficacy of HCQ. Our objective was to systematically review publications and perform a meta-analysis to examine the correlation between HCQ levels and 1) nonadherence and 2) Systemic Lupus Erythematosus Disease Activity Index (SLEDAI) scores, in SLE.

Fully Solution-Processed Transparent Artificial Neural Network Using Drop-On-Demand Electrohydrodynamic Printing.

Artificial neural networks (ANN), deep learning, and neuromorphic systems are exciting new processing architectures being used to implement a wide variety of intelligent and adaptive systems. To date, these architectures have been primarily realized using traditional complementary metal-oxide-semiconductor (CMOS) processes or otherwise conventional semiconductor fabrication processes. Thus, the high cost associated with the design and fabrication of these circuits has limited the broader scientific community from applying new ideas, and arguably, has slowed research progress in this exciting new area.

High-dimensional Stokes vector direct detection over few-mode fibers.

Direct detection attracts much attention for its simplicity compared with coherent detection. In this Letter, we propose for the first time, to the best of our knowledge, a high-dimensional Stokes vector direct detection (HD-SVDD) receiver for mode-division multiplexing transmission in few-mode fibers where the coupled modes can be recovered without resorting to coherent detection. To the best of our knowledge, the first high-dimensional Stokes vector reception based on the proposed HD-SVDD receiver has been successfully demonstrated with a dual-spatial and dual-polarization mode at 60 Gb/s over a 200 m two-mode fiber.

TANK-binding kinase 1 is a mediator of platelet-induced EMT in mammary carcinoma cells.

Platelets can promote several stages of the metastatic process and thus contribute to malignant progression. As an example, platelets promote invasive properties of tumor cells by induction of epithelial to mesenchymal transition (EMT). In this study, we show that tumor necrosis factor receptor-associated factor (TRAF) family member-associated NF-κB activator (TANK)-binding kinase 1 (TBK1) is a previously unknown mediator of platelet-induced EMT in mammary carcinoma cells.

A Photonic Switch Based on a Hybrid Combination of Metallic Nanoholes and Phase-change Vanadium Dioxide.

A photonic switch is an integral part of optical telecommunication systems. A plasmonic bandpass filter integrated with materials exhibiting phase transition can be used as a thermally reconfigurable optical switch. This paper presents the design and demonstration of a broadband photonic switch based on an aluminium nanohole array on quartz utilising the semiconductor-to-metal phase transition of vanadium dioxide.

Differential effect of Platelet Endothelial Cell Adhesion Molecule-1 (PECAM-1) on leukocyte infiltration during contact hypersensitivity responses.

Background: 2'-4' Dinitrofluorobenzene (DNFB) induced contact hypersensitivity is an established model of contact sensitivity and leukocyte migration. Platelet Endothelial Cell Adhesion Molecule-1 (PECAM-1) deficient mice were used to examine the role of PECAM-1 in the migration capacity of several different leukocyte populations after primary and secondary application.

Results: γδ T lymphocytes, granulocytes, and Natural Killer cells were most affected by PECAM-1 deficiency at the primary site of application.

Assessing Brief Changes in Adolescents' Mood: Development, Validation, and Utility of the Fast Assessment of Children's Emotions (FACE).

Mood states of youth have a strong influence on their cooperation, comfort, and engagement in many health care and educational settings. Children who are fearful, angry, or sad are more likely to have difficulty learning new skills or connecting with others. Many interventions are used in hospital and school settings to help youth, but it is difficult to assess their effectiveness without appropriate assessment tools that are easy to administer, age appropriate, and psychometrically sound.

Tonic, but not phasic corticosterone, constrains stress activatedextracellular-regulated-kinase 1/ 2 immunoreactivity within the hypothalamic paraventricular nucleus.

The negative-feedback actions of corticosterone (CORT) depend on both phasic and tonic CORT secretion patterns to regulate hypothalamic-pituitary-adrenal (HPA) axis activity. How these two different CORT secretion pattens influence specific intracellular signal transduction pathway activity within the cellular elements of the HPA axis has not been determined. For example, it is unknown whether CORT has suppressive actions over signal transduction events within medial parvocellular paraventricular nucleus (PVN) corticotrophin-releasing hormone (CRH) neurones, nor whether these suppressive actions are responsible for alterations in PVN transcriptional processes and neurohormone secretion associated with stress.

Flexible low-cost system for small animal aerosol inhalation exposure to drugs, proteins, inflammatory agents, and infectious agents.

The design for a simple, low-cost aerosol generation system for rodent inhalation studies is described here. This system is appropriate for low biohazard-level agents. In this study, two biosafety level 2 agents, Pasturella pneumotropica and Pseudomonas aeruginosa, were tested successfully.