Improvement of the recombinant phytase expression by intermittent feeding of glucose during the induction phase of methylotrophic yeast Pichia pastoris.

Purpose: For growth of methylotrophic yeast, glycerol is usually used as a carbon source. Glucose is used in some cases, but not widely consumed due to strong repressive effect on AOX1 promoter. However, glucose is still considered as a carbon source of choice since it has low production cost and guarantees growth rate comparable to glycerol.

Revealing the formation and electrochemical properties of bis(trifluoromethanesulfonyl)imide intercalated graphite with first-principles calculations.

Graphite has been reported to have anion, as well as cation, intercalation capacities as both a cathode and an anode host material for dual ion batteries. In this work, we study the intercalation of bis(trifluoromethanesulfonyl)imide (TFSI) anions from an ionic liquid electrolyte into graphite with first-principles calculations. We build models for TFSI-Cn compounds with systematically increasing graphene sheet unit cell sizes and investigate their stabilities by calculating the formation energy, resulting in the linear decrease of and arrival at the limit of stability.