Inhibition of hypusine biosynthesis in plasmodium: a possible, new strategy in prevention and therapy of malaria.

The increasing drug resistance of malaria parasites against chemotherapeutics enforces new strategies in finding new drugs. Here, we describe a new class of compounds the piperidone 3-carboxylates which show an antiplasmodial effect in vitro and in vivo. This effect might be caused by inhibition of eukaryotic initiation factor (eIF-5A).

Piperidones with activity against Plasmodium falciparum.

The increasing resistance of the malaria parasites has enforced new strategies of finding new drug targets. We have isolated two genes involved in spermidine metabolism, encoding deoxyhypusine synthase (DHS) and eukaryotic initiation factor 5A (eIF-5A) in the malaria parasites. eIF-5A is activated by the formation of the unusual amino acid hypusine.

Effects of increasing level of supplemental barley on forage intake, digestibility, and ruminal fermentation in steers fed medium-quality grass hay.

Objectives of this research were to evaluate effects of increasing level of barley supplementation on forage intake, digestibility, and ruminal fermentation in beef steers fed medium-quality forage. Four crossbred ruminally cannulated steers (average initial BW = 200 +/- 10 kg) were used in a 4 x 4 Latin square design. Chopped (5 cm) grass hay (10% CP) was offered ad libitum with one of four supplements.

Hospital-physician relationships in the integrated delivery system: an ethical analysis.

The need to compete for managed care contracts is causing hospitals and physicians to dramatically change how they do business with each other. Most significant is the formation of integrated delivery health-care systems. The emergence of these systems creates an enhanced need for physicians and administrators to better understand what the other actually does.

Glycogen levels in human term placental disks, umbilical cords, and membranes.

Glycogen in the placenta and its appendages is important for fetal well-being. The precise location of the glycogen stores, however, is unknown. This study was initiated to quantitate glycogen levels at well-defined sampling sites in more than 641 samples from 10 uncomplicated pregnancies and to correlate these glycogen levels with clinical and morphological variables.

Stress management for the cardiovascular patient: a look at current treatment and trends.

Recent research findings in psychoneurobiology are increasing our clinical knowledge of how emotions and mental stress impact the cardiovascular system. Clinical trial results strongly suggest that morbidity and mortality in cardiac patients can be improved when stress management is a part of a comprehensive treatment plan. This article discusses the state of stress management in current treatment and examines some of the newer interventions and techniques being used to address this risk factor.

Some international efforts of medical schools to improve health care systems.

With growing frequency, medical schools are challenged to redirect their educational and research programs to better respond to the inadequacies of healthcare delivery systems. Some of the accomplishments and difficulties that may arise when this is attempted can be found in the experience of three new schools linked to large healthcare systems in different areas of the world.

Influence of Aspergillus oryzae fermentation extract on forage intake, site of digestion, in situ degradability, and duodenal amino acid flow in steers grazing cool-season pasture.

Ten ruminally and duodenally cannulated (326 +/- 28 kg) and four esophageally fistulated (394 +/- 23 kg) steers grazing cool-season pasture throughout the growing season were used to evaluate the influence of Aspergillus oryzae fermentation extract (AO) supplementation on intake, forage nutrient utilization, and duodenal amino acid flow. Steers grazed a predominantly smooth brome (Bromus inermis L.) pasture, and measurements were taken in three periods (June, July, and August).

Special needs of the young spinal cord injured patient in a nursing home.

Discharge plans for some spinal cord individuals may include nursing home placement. Since many spinal cord injured patients are young males, what special needs are expressed by this patient population? To determine specific concerns from a patient's perspective, structured interviews were conducted with 15 spinal cord injured patients under 35 years of age. Special needs were organized into six major categories: physical locations, staff interactions, nutrition, physical therapy, social activities, and sexuality.

Effect of discontinuance of type A behavioral counseling on type A behavior and cardiac recurrence rate of post myocardial infarction patients.

Three hundred post infarction participants who had received type A behavioral counseling in the Recurrent Coronary Prevention Project, (RCPP) agreed to be followed for 1 additional year after stopping 4.5 years of continuous type A behavioral counseling. One hundred fourteen participants who had served for 4.

Alteration of type A behavior and its effect on cardiac recurrences in post myocardial infarction patients: summary results of the recurrent coronary prevention project.

One thousand thirteen post myocardial infarction patients were observed for 4.5 years to determine whether their type A (coronary-prone) behavior could be altered and the effect such alteration might have on the subsequent cardiac morbidity and mortality rates of these individuals. Eight hundred sixty-two of these individuals were randomly assigned either to a control section of 270 participants who received group cardiac counseling or an experimental section of 592 participants who received both group cardiac counseling and type A behavioral counseling.

Campylobacter jejuni septicemia--epidemiology, clinical features and outcome.

In 33 cases of Campylobacter jejuni septicemia, the disease was more common at the extremes of age: infants made up a third of the reported cases while 24% of patients were older than 50 years. Fever was noted in more than 80% of patients and chills in about a fourth. Enteritis was present in 70% of cases, and the gastrointestinal tract was the principal source of septicemia.

Induction of Cellulases in chaetomium cellulolyticum by cellobiose.

The production of extracellular cellulases by Chaetomium cellulolyticum could be induced by slow feeding of cellobiose to the cultures. Both the rate of production and the amount of activity were comparable to that obtained in batch cultivation on cellulose. The specific filter paper activity of 2.

Reduction in type A behavior in healthy middle-aged American military officers.

One hundred eighteen senior officer-students of the U.S. Army War College who were healthy but exhibited type A behavior volunteered to be randomly selected and enrolled into (1) a section of 62 officers who received group type A behavior counseling for 9 months and (2) a control section of 56 officers who received no counseling of any kind.

Societal influences on health and life-styles.

Strong sociocultural forces affect individual attitudes toward health and choice of life-style. Economic deprivation fosters negative health behaviors. Positive health habits are reinforced by discrete societal groups.

Use of avidin to prepare biotin-free culture media.

Dialysis of avidin-treated casein hydrolysate and peptone solutions produced biotin-free dialysates which, when incorporated into growth media resulted in media devoid of biotin. Wright-Skeggs medium supplemented with dialyzed, avidin-treated, casein hydrolysate did not support significant growth of Bacillus coagulans or Lactobacillus plantarum without biotin supplementation. Eagle's minimal essential medium supplemented with avidin-treated, peptone dialysate (MEMAPD) would not support the growth of LM strain mouse cells through more than a single passage without biotin supplementation.

Alteration of type A behavior and reduction in cardiac recurrences in postmyocardial infarction patients.

Eight hundred sixty-two postmyocardial infarction patients volunteered to be randomly selected and enrolled into: (1) a control section of 270 patients, who received group cardiologic counseling; and (2) an experimental section of 592 patients, who received group type A behavior counseling in addition to group cardiologic counseling. Reduction in type A behavior at the end of 3 years was observed in 43.8% of the 592 participants, who initially were enrolled to receive group cardiologic and type A behavioral counseling.