The Assessment of Methyl Methanesulfonate Absorption by Amphipods from the Environment Using Lux-Biosensors.

The ability of aquatic mesofauna representatives involved in trophic chains to sorb and accumulate toxicants is important for understanding the functioning of aquatic ecosystems and for fishing industry. This study investigated the capacity of marine amphipod and freshwater amphipods and to absorb the DNA-alkylating agent methyl methanesulfonate (MMS). The presence of alkylating agents in the environment and in the tissues of the amphipods was determined using whole-cell lux-biosensor MG1655 pAlkA-lux, in which the genes from , enabling the luminescence of the cell culture, are controlled by the P promoter of DNA glycosylase.

Observation of Cytotoxicity of Phosphonium Derivatives Is Explained: Metabolism Inhibition and Adhesion Alteration.

The search for new antibiotics, substances that kill prokaryotic cells and do not kill eukaryotic cells, is an urgent need for modern medicine. Among the most promising are derivatives of triphenylphosphonium, which can protect the infected organs of mammals and heal damaged cells as mitochondria-targeted antioxidants. In addition to the antioxidant action, triphenylphosphonium derivatives exhibit antibacterial activity.

Genotoxic Effect of Dicyclopropanated 5-Vinyl-2-Norbornene.

Dicyclopropanated 5-vinyl-2-norbornene (dcpVNB) is a strained polycyclic hydrocarbon compound with a high energy content, which makes it promising for the development of propellant components based on it. In this work, the genotoxic properties of dcpVNB were studied using whole-cell -biosensors based on and . It was shown that the addition of dcpVNB to bacterial cells leads to the appearance of DNA damage inducing the SOS response and Dps expression with slight activation of the OxyR-mediated response to oxidative stress.

Anti-Inflammatory Activity of Soluble Epoxide Hydrolase Inhibitors Based on Selenoureas Bearing an Adamantane Moiety.

The inhibitory potency of the series of inhibitors of the soluble epoxide hydrolase (sEH) based on the selenourea moiety and containing adamantane and aromatic lipophilic groups ranges from 34.3 nM to 1.2 μM.

Constructing of -Based Lux-Biosensors with the Use of Stress-Inducible Promoters.

Here, we present a new lux-biosensor based on for detecting of DNA-tropic and oxidative stress-causing agents. Hybrid plasmids pNK-DinC, pNK-AlkA, and pNK-MrgA have been constructed, in which the reporter genes are transcribed from the stress-inducible promoters of : the SOS promoter P, the methylation-specific response promoter P, and the oxidative stress promoter P. The luminescence of based biosensors specifically increases in response to the appearance in the environment of such common toxicants as mitomycin C, methyl methanesulfonate, and HO.

Influence of the Regulatory Gene Dosage and Expression Level on the Sensitivity of the Whole-Cell Biosensor to Acyl-Homoserine Lactone.

LuxR and LuxR1 and LuxR2 regulatory proteins are quorum sensing transcriptional (QS) activators, inducing promoters of genes in the presence of an autoinducer (3-oxo-hexanoyl-l-homoserine lactone). In the cells, genes are regulated by HNS, CRP, LitR, etc. Here we investigated the role of the expression level in LuxI/R QS system functionality and improved the whole-cell biosensor for autoinducer detection.