[Consumption of volatile organic compounds by alcaliphilic microorganisms].

It has been shown that various microbial species used in bioreactors for purification of air from volatile organic compounds can grow at alkaline pH values consuming the xenobiotics as sole carbon sources. The alkali tolerance depends on the carbon source. The alkaline pH of the medium reduces the foreign microbial population restricting the potential of the bioreactor.

[Search for yeast producers of brassylic and sebacic fatty acids].

Yeast cultures belonging to the genera Candida, Torulopsis, Saccharomyces, Debaryomyces, Hansenula, Pichia, and Yarrowia, capable of synthesizing brassylic and sebacic fatty acids, were screened. Overall, about 200 cultures grown in media containing decane or tridecane as a sole source of carbon were tested. On the medium with tridecane, yeasts synthesized insignificant amounts of brassylic acid.

[Analysis of kinetic parameters of gas phase bioreactors].

The dependence of toluene elimination capacity on its load was obtained in five small-scale reactors filled with glass beads carrying biocatalyst cells. With increase in operation time the calculated maximal elimination capacity was shown to increase along with biomass density in the biocatalyst bed. Fivefold increase in trickling intensity did not affect the reactor performance.

[Beta-galactosidase of the superthermophilic anaerobic bacteria Thermoanaerobium sp. 2905 and its immobilization].

The composition of nutrition medium for cultivating the extreme thermophilic anaerobe Thermoanaerobium sp. 2905 was optimized, which enabled the bacterial beta-galactosidase production to be substantially enhanced. Xylan and ammonium phosphate were selected as optimal carbon and nitrogen sources, respectively.

[Furane compounds formed upon heating highly-sugared fermented media].

5-Methylfurfural diethyl acetal, 5-methylfuran acid ester, and 4-hydroxypentanoic acid ethyl ester were first identified in high-sacchariferous corn media subjected to alcoholic fermentation by Mucor X-I culture and in aged sweet wines by means of GLC, GLC-MS, and TLC, as well as by UV and IR spectroscopic examinations. These substances are products of enzymatic esterification of sugars followed by their dehydration by heat treatment or long seasoning of grape wines.

Structure and properties of a Cephalosporium acremonium alpha-galactosidase.

The amino acid and sugar composition of the enzyme protein, the effect of urea, sodium dodecyl sulphate and Concanavalin A on the purified alpha-galactosidase (EC 3.2.1.

Low-molecular-weight trypsin inhibitors of microbial origin.

Three hundred actinomyces cultures newly isolated from the soil of different regions of the Soviet Union were tested for their ability to produce inhibitors of trypsin-like proteases. Seven previously not known to produce trypsin inhibitors (Streptomyces bikiniensis 17-5, S. sporoclivatus 28-1, S.

[Actinomycetes producing trypsin inhibitors].

The ability to produce inhibitors of trypsin-like proteases was tested in 300 cultures of actinomycetes freshly isolated from different soils of the USSR. A high antitrypsin activity was found in seven cultures which had not been known before as those producing trypsin inhibitors: Streptomyces sporoclivatus 28 (1), S. lavendulae 29 (4), S.

[Isolation and properties of an alpha-galactosidase preparation from Cephalosporium sp. 237].

Different methods for preparing alpha-galactosidase from the culture fluid of the micromycete Cephalosporium sp. 237 were tested. They included precipitation with ethanol, isopropanol, and acetone.

[Microbial alpha-galactosidase (a review)].

The review discusses properties, distribution and potential use of microbial alpha-galactosidase (alpha-D-galactoside galactohydrolase, EC 3.2.1.

[Purification of glucosoisomerase from Lactobacillus brevis and its physico-chemical properties].

Glucosoisomerase isolated from disrupted cells of Lactobacillus brevis 74 by extraction with K, Na phosphate buffer was purified by step-by-step fractionation with (NH4)2SO4 and DEAE-cellulose chromatography. The purified enzyme was examined by polyacrylamide gel electrophoresis and ultracentrifugation. The examination showed the enzyme to be homogeneous.

[Amino acid composition and quaternary structure of glucosoisomerase (d-xylose-ketol-isomerase) from Actinomyces olivocinereus 154].

The amino acid composition of glucosoisomerase from Actinomyces olivocinereus 154 was investigated. The content of dicarboxylic acids--aspartic and glutamic--was found to be greater than that of basic acids--lysine, arginine and histidine. Hydrophobic acids were also detected to occur on appreciable quantities.

[Inhibition of glucose isomerase from Actinomyces olivocinereus 154 by polyols].

The inhibitory action of polyols, e. g. D-mannitol, D-glucitol, ribitol and xylitol, on the activity of glucose isomerase prepared from Actinomyces olivocinereus 154 was studied.

[Conversion of D-glucose into D-fructose in a column reactor with immobilized glucose isomerase].

Conversion of D-glucose into D-fructose in a column reactor of a continuous type was studied using an immobilized enzyme, glucose isomerase from Actinomyces olivocinereus. The effective parameters of Km, Vmax, energy activation at various flow rates were calculated. During 32 days of continuous operation the enzyme activity fell down to 84% of the initial level.

[Alpha-galactosidase producers among yeast cultures].

The capacity for biosynthesis of alpha-galactosidase was examined in 89 yeast cultures belonging to 21 genera during submerged cultivation on a medium containing dry whey. This capacity was found only in one genus, namely Schwanniomyces. Optimal cultivation conditions were selected for the strain Schw.

[Highly purified glucosoisomerase from Actinomyces olivocinereus 154].

The paper presents a method for producing highly purified glucosoisomerase from Actinomyces olivocinereus 154. The scheme of purification includes enzyme extraction from dry biomass, acetone fractionation, ammonium sulfate precicipation, and Sephadex G-200 chromatography. The highly purified enzyme is homogeneous as shown by polyacryl amide gel electrophoresis, analytical ultracentrifugation, and gel filtration.

[Microorganisms that produce alpha-galactosidase, alpha-mannosidase, alpha-fucosidase and beta-acetylglucosaminidase].

The capacity to synthesize alpha-D-galactosidase (EC 3.2.1.

[Glucose-isomerizing enzyme from Actinomyces olivocinereus and its immobilization on aminosilochrome].

Glucose-isomerizing enzyme was isolated from the culture of Actinomyces olivocinereus. It was purified by the chromatography on DEAE-cellulose. The samples of glucose-isomerasing enzyme are homogeneous according to the results of analytical electrophoresis and ultracentrifugation.

[Study of oxidative enzymes of the lignin-degrading fungus Pleurotus ostreatus].

The elctrophoretic separation in polyacrylamide gel of laccase and peroxidase isoenzymes of the lignin-degrading fungus Pleurotus ostreatus was investigated. The optimal electrophoretic conditions were found: in the electrode buffer tris-diethyl barbituric acid pH 7.0 in the gradient gel-4-10% acrylamide.