Toxicity screening of mouthwashes in the pollen tube growth test: safety assessment of recommended dilutions.

This study investigated whether mouthwash dilutions, recommended by the manufacturers, may contribute to oral mucosa irritation. Twenty frequently used mouthwash brands were examined by the pollen tube growth test, an alternative in vitro method for assessment of the irritating potential of ingredients of cosmetic formulations. The test is based on the photometric quantification of pollen tube growth inhibition.

Toxicity screening of mouthwashes in the pollen tube growth test: safety assessment of recommended dilutions of twenty brands.

The question of whether mouthwash dilutions, recommended by the producers, contribute to irritation of the oral mucosa was examined. Twenty frequently used mouthwash brands were examined by the pollen tube growth test, an alternative in vitro method for the irritation assessment of ingredients of cosmetic formulations. The test is based on the photometric quantification of pollen tube growth inhibition.

Toxicity assessment of mouthwashes in the pollen tube growth test.

Aim: Mouthwashes are frequently used to treat oral discomfort, halitosis or superficial infections. Adverse effects of mouthwash use are recognized, e.g.

Morphology of cell injury: An approach to the EDIT programme by the use of tobacco pollen tubes. Evaluation-guided Development of New In Vitro Test Batteries.

Tobacco pollen tubes were used as a standard in vitro system to investigate cell growth aberrations caused by some of the Multicentre Evaluation of In Vitro Cytotoxicity (MEIC) programme chemicals and other toxic compounds. Changes in cytoskeletal pattern were observed in the tube cells by using tubulin immunofluorescence and rhodamin-phalloidin fluorescence for the localisation of microtubules and actin filaments, respectively. Four different types of cell malformation were found: screw-like growth, isodiametric tip swelling, hook formation, and pollen grain enlargement.

Development of an in vitro test battery for the estimation of acute human systemic toxicity: An outline of the EDIT project. Evaluation-guided Development of New In Vitro Test Batteries.

The aim of the Evaluation-guided Development of new In Vitro Test Batteries (EDIT) multicentre programme is to establish and validate in vitro tests relevant to toxicokinetics and for organ-specific toxicity, to be incorporated into optimal test batteries for the estimation of human acute systemic toxicity. The scientific basis of EDIT is the good prediction of human acute toxicity obtained with three human cell line tests (R(2) = 0.77), in the Multicentre Evaluation of In Vitro Cytotoxicity (MEIC) programme.

Adaptation of the Pollen Tube Growth Assay to Cytotoxicity Testing of Cigarette Smoke.

We describe a recently developed in vitro assay for the assessment of the cytotoxicity of gaseous materials. The method is based on the pollen tube growth (PTG) test suited to determine the cytotoxicity of non-gaseous, water-soluble substances (Kristen and Kappler, 1995). Instead of the conventional PTG test, we used a semi-automatic, computer-controlled multi-channel device for the injection of gases and gas mixtures into a battery of incubation tubes containing germinating, suspension-cultured tobacco pollen as indicator of toxic effects.