Publications by authors named "Udhayabhaskar Sathyanarayanan"
G proteins are universally important for signal transduction in mammalian cells. The underlying kinetics and transformation from extracellular stimuli into intracellular signaling, however could not be investigated in detail so far. Here we present the human Neuropsin (hOPN5) for specific and repetitive manipulation of G signaling in vitro and in vivo with high spatio-temporal resolution.
View Article and Find Full Text PDFSigns of proteostasis failure often entwine with those of metabolic stress at the cellular level. Here, we study protein sequestration during glucose deprivation-induced ATP decline in Saccharomyces cerevisiae. Using live-cell imaging, we find that sequestration of misfolded proteins and nascent polypeptides into two distinct compartments, stress granules, and Q-bodies, is triggered by the exhaustion of ATP.
View Article and Find Full Text PDFArticle Synopsis
- The nervous and neuroendocrine systems depend on quickly regulated release of neurotransmitters via a process called SNARE-mediated exocytosis, which involves specific proteins.
- The study focuses on amisyn, a protein that negatively regulates this exocytosis by forming a complex with other proteins like syntaxin-1 and SNAP-25, which disrupts the assembly needed for neurotransmitter release.
- Amisyn's function relies on both its SNARE motif and an N-terminal pleckstrin homology domain, indicating its role in inhibiting exocytosis is linked to its interactions with vesicles and the cell membrane, affecting vesicle priming rather than fusion.