Detection of Theileria in cattle ticks (Rhipicephalus microplus) (Canestrini, 1888) in upper-northeastern Thailand.

Theileria parasites were investigated from cattle ticks (Rhipicephalus microplus (Canestrini, 1888)) collected in 12 provinces in upper-northeastern Thailand based on the sequences of 18S rRNA and MPSP gene. Polymerase chain reactions (PCRs) and sequencing specific regions for the 18S rRNA gene revealed two species of Theileria pathogens; T. orientalis (n = 42) and T.

Direct Observation of Feeding Behavior of Adult Tabanidae (Diptera) on Beef Cattle from Khon Kaen Province in Thailand.

Tabanidae (horse flies and deer flies) are hematophagous insects that cause direct and indirect damage to animal production. The aims of this study were to determine the preferred site, time of day, and duration of tabanid feeding on beef cattle and identify factors related to infestation by tabanids. The population of tabanids was surveyed on certain body parts of the beef cattle (fore udder, tail, navel, leg, dewlap, body, and under) during the morning hours (9.

Diversity, Distribution and Host Blood Meal Analysis of Adult Black Flies (Diptera: Simuliidae) from Thailand.

Understanding the factors associated with the species diversity and distribution of insect vectors is critically important for disease epidemiology. Black flies (Diptera: Simuliidae) are significant hematophagous insects, as many species are pests and vectors that transmit pathogens to humans and other animals. Ecological factors associated with black fly species distribution have been extensively examined for the immature stages but are far less well explored for the adult stage.

Characterization of the bacterial microbiota of cattle ticks in northeastern Thailand through 16S rRNA amplicon sequencing.

Ticks are vectors of a variety of pathogens that can infect humans and animals. Ticks also harbor non-pathogenic microbiota. This study characterized the microbiota of the ticks infesting beef cattle in Thailand.

Genetic diversity of tick (Acari: Ixodidae) populations and molecular detection of Anaplasma and Ehrlichia infesting beef cattle from upper-northeastern Thailand.

Genetic diversity, genetic structure and demographic history of the ticks infesting beef cattle in Thailand were examined based on mitochondrial cytochrome c oxidase I (cox1) sequences. Tick samples were collected in 12 provinces in upper-northeastern Thailand. Three species were found; Rhipicephalus microplus, R.

Effect of Larval Diets on House Fly (Diptera: Muscidae) Production and Host Suitability for a Pupal House Fly Parasitoid (Spalangia gemina) (Hymenoptera: Pteromalidae).

A larval medium for house fly (Musca domestica L.) suitable for house fly parasitoid production was developed from locally available and inexpensive food ingredients. Biological parameters and life table parameters were estimated for house flies treated with five different diets.

Seasonal population dynamics and a role as natural vector of Leucocytozoon of black fly, Simulium chumpornense Takaoka & Kuvangkadilok.

The black fly, Simulium chumpornense Takaoka & Kuvangkadilok, is suspected to be a natural vector of Leucocytozoon. In this study, seasonal variation in abundance of the adult fly and molecular detection of Leucocytozoon in S. chumpornense and in domestic chicken was investigated in northeastern Thailand.

Molecular identification of blood meal sources in black flies (Diptera: Simuliidae) suspected as leucocytozoon vectors.

Identification of the host blood meal of hematophagous insects can provide significant information regarding host preferences and the possibility of the transmission of disease agents. Currently, this knowledge is limited for black flies in the Oriental region. In this study, we used cytochrome b gene sequences for identification of blood meal sources of two black fly taxa, the Simulium asakoae complex and S.

Molecular detection of Trypanosoma (Kinetoplastida: Trypanosomatidae) in black flies (Diptera: Simuliidae) from Thailand.

Trypanosomes are parasitic flagellate protozoans that are important disease causing agents in humans and animals including economically significant livestock. Many blood-sucking insects are known to be vectors of trypanosomes but there is no data for the haematophagous black fly species in Asia. In this study, a molecular approach based on the small subunit ribosomal RNA (SSU rRNA) gene was used to detect trypanosomes in black flies from Thailand.

Who is biting you? DNA barcodes reveal cryptic diversity in human-biting black flies (Diptera: Simuliidae).

Black flies (Simuliidae) are important biting insects and vectors of diseases agents of humans and livestock. Thus, understanding the taxonomy and biodiversity of these insects is crucial for control and management of these diseases. In this study, we used mitochondrial cytochrome c oxidase I sequences to examine genetic diversity of three human-biting and possible vector black fly taxa; the Simulium asakoae species-complex, S.

Molecular detection of Leucocytozoon (Apicomplexa: Haemosporida) in black flies (Diptera: Simuliidae) from Thailand.

Information regarding vector-parasite association is necessary for fully understanding the epidemiology of vector borne diseases yet, this information is lacking in the case of Leucocytozoonosis in the Oriental region, despite a high incidence of the disease. In this study, we used a molecular approach based on mitochondrial cytochrome b (cyt b) sequence to detect the parasite, Leucocytozoon, in potential black fly (Simuliidae) vectors in Thailand. A total of 404 wild caught black flies representing six morphological species of two subgenera were examined.

A new black fly (Diptera: Simuliidae) species of the subgenus Asiosimulium Takaoka Choochote from Thailand.

A new black fly species of the subgenus Asiosimulium Takaoka Choochote of the genus Simulium was recognized from northeastern Thailand based on morphology, mitochondrial DNA and ecology. This black fly species has similar morphological characteristics to Simulium oblongum Takaoka and Choochote that was also described from the same geographic region in all life stages. However, this new species could be distinguished at the adult stage by coloration of the maxillary palp and in the larval stage by the presence of a pigmented subesophageal ganglion that is lacking in S.