The kinetics of DNAse inhibition by specific antibodies.

The inhibition of beef pancreatic deoxyribonuclease I activity with specific antibodies is studied using the hypochromic shift of DNA at 260 nm. The kinetics of this reaction reveals two reaction phases. The first phase, which ends within seconds, is reversible and follows second order kinetics.

Clinical value of 14C-phenylacetic oil as a fat absorption test.

The value of [1-14C] phenylacetic oil as a clinical test for malassimilation was evaluated in 20 normal volunteers, 20 patients with various non-gastrointestinal illnesses, 10 patients with malabsorption, and 7 patients with maldigestion. All subjects were given a test meal composed of 0.5 ml of 14C-phenylacetic oil (0.

Purification and biochemical characterization of human lymphocyte mitogenic factor (LMF).

Supernatants of human T lymphocytes stimulated by TT antigen release two factors that induce mitogenesis in autologous and allogeneic B lymphocytes. These factors are precipitated by 60% ammonium sulfate and 50% ethanol, and are both destroyed by heating to 70 degrees C for 5 min. By equilibrium ultracentrifugation there was a peak of mitogenic activity in the fraction with a specific gravity of 1.