Publications by authors named "U T Timur"
Article Synopsis
- The research aimed to compare the chondrocyte signaling profiles between non-osteoarthritic and end-stage osteoarthritic knee synovial fluid to understand their effects on cartilage cells.
- Protein profiling techniques were employed to analyze the different signaling patterns and their consequences on chondrocyte behavior.
- Findings revealed that osteoarthritic synovial fluid has more inflammatory cytokines and growth factors, leading to altered signaling that promotes abnormal chondrocyte behavior and contributes to cartilage degeneration.
Objective: The potential chondroprotective effect of celecoxib, a nonsteroidal anti-inflammatory drug and selective cyclooxygenase-2 inhibitor used to reduce pain and inflammation in knee osteoarthritis patients, is disputed. This study aimed at investigating the chondroprotective effects of celecoxib on (1) human articular cartilage explants and (2) in an osteoarthritis rat model.
Design: Articular cartilage explants from 16 osteoarthritis patients were cultured for 24 hours with celecoxib or vehicle.
Increasing extracellular osmolarity 100 mOsm/kg above plasma level to the physiological levels for cartilage induces chondrogenic marker expression and the differentiation of chondroprogenitor cells. The calcineurin inhibitor FK506 has been reported to modulate the hypertrophic differentiation of primary chondrocytes under such conditions, but the molecular mechanism has remained unclear. We aimed at clarifying its role.
View Article and Find Full Text PDFBackground: Focal cartilage defects are often debilitating, possess limited potential for regeneration, are associated with increased risk of osteoarthritis, and are predictive for total knee arthroplasty. Cartilage repair studies typically focus on the outcome in younger patients, but a high proportion of treated patients are 40 to 60 years of age (ie, middle-aged). The reality of current clinical practice is that the ideal patient for cartilage repair is not the typical patient.
View Article and Find Full Text PDFIdentification of tissue-dependent proteins in knee OA synovial fluid.
Osteoarthritis Cartilage
January 2021
Objective: For many proteins from osteoarthritic synovial fluid, their intra-articular tissue of origin remains unknown. In this study we performed comparative proteomics to identify osteoarthritis-specific and joint tissue-dependent secreted proteins that may serve as candidates for osteoarthritis biomarker development on a tissue-specific basis.
Design: Protein secretomes of cartilage, synovium, Hoffa's fat pad and meniscus from knee osteoarthritis patients were determined using liquid chromatography tandem mass spectrometry, followed by label-free quantification.