Targeted Assay Engineering Enhances Bile Salt Hydrolase Activity in Heyndrickxia coagulans ATCC 7050 and Lactiplantibacillus plantarum ATCC 10012.

The recent emergence of bile salt hydrolase (BSH) enzyme as a therapeutic target reflects its unbound potential in mitigating hypercholesterolemia, obesity, and gastrointestinal issues. However, to bolster its industrial application, optimization of BSH assay lays the cornerstone for enhancing sensitivity, specificity, and reproducibility. The current study delved into optimizing the BSH assay parameters utilizing response surface methodology (RSM) and one-factor-at-a-time (OFAT) method for two novel, natural BSH producers, Heyndrickxia coagulans ATCC 7050 and Lactiplantibacillus plantarum ATCC 10012.

Ultrasound assisted osmotic dehydration of sweet lime (Citrus limetta) slices: Process optimization and mass transfer kinetics.

Sweet lime (Citrus limetta), known for its unique taste and aroma, is limited in use due to its bitterness. Osmotic dehydration of sweet lime slices was studied to optimize mass transfer kinetics using response surface methodology (RSM). The debittering pre-treatment using NaCl ranging from 0 to 5 % resulted in a significant reduction of bittering compounds, achieving a 39.

Assessing the impact of pin-to-plate atmospheric cold plasma on activity, stability, kinetics, and conformation of α-amylase.

α-amylase is responsible for shortening the shelf life of food by degrading starch and glycogen into maltose, disaccharides, and oligosaccharides. This study focuses on the effect of atmospheric cold plasma on the activity, stability, enzyme kinetics, and structural change for its application in α-amylase modifications. A reduction in the residual activity from 96.

Atmospheric pin-to-plate cold plasma modification of amaranth starch & its application as a stabilizer in low-fat mayonnaise.

This study investigates the changes in physicochemical, functional, rheological, and structural characteristics of the amaranth seed starch upon atmospheric cold plasma exposure with the generation/input voltages of 170, 200, and 230 V for 5-15 min and its potential as a fat replacer in a model emulsion system (mayonnaise). The surface modification by cold plasma is expected to enhance the native amaranth starch characteristics. Plasma treatment reduced the amylose content to a minimum of 9.

Fermentation dynamics of bile salt hydrolase production in Heyndrickxia coagulans ATCC 7050 and Lactiplantibacillus plantarum ATCC 10012: Addressing ninhydrin assay limitations with a novel HPTLC-MS method.

Bile salt hydrolase (BSH), a pivotal enzyme in cholesterol management, holds significant promise in both human and animal subjects. This study investigated the effect of fermentation dynamics in Heyndrickxia coagulans ATCC 7050 and Lactiplantibacillus plantarum ATCC 10012 to enhance BSH production. Cultivation of cultures in MRS and M17 media revealed that MRS medium enhanced BSH production by 235.