Hepatic metabolic clearance is one of the most important factors driving the overall kinetics of chemicals including substances used in various product categories such as pesticides, biocides, pharmaceuticals, and cosmetics. A large number of in vitro systems from purified isozymes and subcellular organelles to hepatocytes in simple cultures and in complex scaffold setups are available for measuring hepatic metabolic clearance for different applications. However, there is currently no approach for systematically characterising and comparing these in vitro methods in terms of their design, applicability and performance.
View Article and Find Full Text PDFThe influence of preoperative radiotherapy on the prevalence of DNA aneuploidy and the prognostic significance of tumour DNA ploidy was evaluated in 126 patients with squamous cell carcinoma of the oesophagus. Preoperative radiotherapy with 30 Gy was performed in 52 patients. DNA ploidy was analysed by flow cytometry on nuclei isolated from paraffin embedded tumour tissue.
View Article and Find Full Text PDFBackground: The prognostic significance of tumor DNA ploidy in patients with cancer of the pancreas has not been defined because conflicting results have been reported.
Methods: DNA content was measured in 56 ductal adenocarcinomas of the pancreas. DNA ploidy status was evaluated by flow cytometry in nuclei isolated from paraffin-embedded tumor tissues.