Dynamic behavior of different quantities of osteoblasts during formation of micromass cultures.

Implantation of micromass cultures of osteoblastic cells offers the possibility of scaffold free tissue engineering for example, regeneration of bone defects. However, the details of cell dynamics during the formation of these micromasses are still not well understood. This study aims to investigate and clarify the extent to which cell quantity influences the dynamics of micromass formation of osteoblastic cell cultures.

Quantitative analysis of dynamic behavior of osteoblasts during in vitro formation of micro-mass cell cultures.

Improvements in bone tissue engineering require an understanding of cellular and tissue level behavior of osteoblast-like cells. Experiments indicate that in the absence of an anchoring material, intercellular adhesion may be based on signals that promote cell activity resulting in the formation of a spheroid cell-matrix. The aim of the present study is to investigate the formation of scaffold-free three-dimensional micro-mass cell spheroids in vitro, and to characterize quantitatively the cell movement.

An in vitro study of osteoblast vitality influenced by the vitamins C and E.

Vitamin C and vitamin E are known as important cellular antioxidants and are involved in several other non-antioxidant processes. Generally vitamin C and vitamin E are not synthesized by humans and therefore have to be applied by nutrition. The absence or deficiency of the vitamins can lead to several dysfunctions and even diseases (e.

Kinetic oxygen measurements by CVC96 in L-929 cell cultures.

Generally animal and human cells use oxygen during their whole life. Consequently the oxygen use is a simple indicator to test the vitality of cells. When the vitality decreases by the delivery of toxic substances the decrease can be observed directly by the oxygen-use of the cells.