Decoding the physicochemical basis of resurrection: the journey of lichen Flavoparmelia caperata through prolonged water scarcity to full rehydration.

Desiccation tolerance is a complex phenomenon observed in the lichen Flavoparmelia ceparata. To understand the reactivation process of desiccated thalli, completely dried samples were rehydrated. The rehydration process of this lichen occurs in two phases.

Building machines that learn and think with people.

What do we want from machine intelligence? We envision machines that are not just tools for thought but partners in thought: reasonable, insightful, knowledgeable, reliable and trustworthy systems that think with us. Current artificial intelligence systems satisfy some of these criteria, some of the time. In this Perspective, we show how the science of collaborative cognition can be put to work to engineer systems that really can be called 'thought partners', systems built to meet our expectations and complement our limitations.

High-resolution visualisation of antisense oligonucleotide release from polymers in cells.

Antisense oligonucleotides (ASOs) are a well-established therapeutic modality based on RNA interference, but low cellular uptake, limited ability to direct ASO trafficking, and a range of intracellular barriers to successful activity compromise both gene silencing outcomes and clinical translations. Herein, we demonstrate that polymers can increase ASO internalisation intracellular trafficking pathways that are distinct from lipid-based delivery reagents. For the first time, we spatially define internalisation and dissociation stages in the polymer-mediated cytosolic delivery of ASOs using Nanoscale Secondary Ion Mass Spectrometry (NanoSIMS), which enables visualisation of ASO localisation at the organelle level.