[Animal and viral mRNA translation in a mRNA-dependent cell-free system and rabbit reticulocytes].

A system of cell-free synthesis from rabbit reticulocytes is described. A method of repeated bloodlettings for induction of reticulocytosis was used. The lysate of washed reticulocytes was treated with micrococcus nuclease enabling effective inhibition of synthesis in endogenous templates.

[Translation of mouse interferon messenger RNA in a cell-free system].

Crude mouse interferon mRNA extracted from poly (I) . poly (C)-induced L929 cells has been translated in a cell-free system from rabbit reticulocytes and in two-cell systems--L929 cells and chick embryo fibroblasts. Translational efficiency of interferon mRNA preparation was 100-fold higher in the cell-free system than in tissue culture cells.

Comparative analysis of interferon and antiviral protein messenger RNAs.

A comparative analysis of interferon and antiviral protein messenger RNAs was carried out. Differences in their biological activities and sedimentation coefficients were found. In RNA preparations from superinduced cells (cells treated with poly(I).