Publications by authors named "U K RoeSSler"
The Running the European Network of biological and retrospective dosimetry (RENEB) network of laboratories has a range of biological and physical dosimetry assays that can be deployed in the event of a radiation incident to provide exposure assessment. To maintain operational capability and provide training, RENEB runs regular inter-laboratory comparison (ILC) exercises. The RENEB ILC2021 was carried out with all the biological and physical dosimetry assays employed in the network.
View Article and Find Full Text PDFPurpose: X-ray cabinets are replacing Cs/ Co sources in radiation biology research due to advantages in size, handling, and radiation protection. However, because of their different physical properties, X-ray cabinets are more susceptible to experimental influences than conventional sources. The aim of this study was to examine the variations related to the experimental setups typically used to investigate biological radiation effects with X-ray cabinets.
View Article and Find Full Text PDFAnalysis of chromosomal aberrations and γH2A.X foci to identify radiation-sensitive ataxia-telangiectasia patients.
Mutat Res Genet Toxicol Environ Mutagen
March 2021
Article Synopsis
- - Ataxia-telangiectasia (AT) is a rare genetic disorder caused by a mutation in the ATM gene, leading to issues like chromosomal instability, cancer risk, and heightened sensitivity to radiation.
- - The study examined DNA damage and repair in lymphocytes from 8 AT patients and 10 healthy individuals after radiation exposure, finding varied responses among patients regarding γH2A.X foci and DNA repair capacity.
- - Results indicated that while γH2A.X foci may not reliably indicate radiation sensitivity due to individual mutation differences, complex chromosomal aberrations and dicentric chromosomes could serve as effective biomarkers for assessing radiation sensitivity in AT patients.
Article Synopsis
- Fast and accurate dose assessment is essential for treating individuals exposed to ionizing radiation and alleviating concerns for those who are not significantly affected.
- The study trained inexperienced operators to perform the γH2A.X radiation-induced foci assay and analyze blood samples, comparing their results to expert assessments.
- Findings indicate that with proper training, inexperienced staff can effectively assist in slide preparation and manual foci scoring, aiding in timely triage during large-scale radiation exposure incidents.
Purpose: To conduct a feasibility study on the application of the γ-H2AX foci assay as an exposure biomarker in a prospective multicentre paediatric radiology setting.
Materials And Methods: A set of in vitro experiments was performed to evaluate technical hurdles related to biological sample collection in a paediatric radiology setting (small blood sample volume), processing and storing of blood samples (effect of storing blood at 4°C), the reliability of foci scoring for low-doses (merge γ-H2AX/53BP1 scoring), as well as the impact of contrast agent administration as potential confounding factor. Given the exploratory nature of this study and the ethical constraints related to paediatric blood sampling, blood samples from adult volunteers were used for these experiments.