Publications by authors named "U Grossniklaus"

Article Synopsis
  • Whole-genome bisulfite sequencing (WGBS) is commonly used for analyzing DNA methylation across genomes, but the bisulfite treatment often leads to DNA degradation.
  • To address this, the post-bisulfite adapter tagging (PBAT) method was developed, which adds adapters after the bisulfite treatment.
  • PBAT facilitates single-cell bisulfite sequencing (scBS-seq), allowing researchers to study DNA methylation with minimal DNA from just a few cells, making the process efficient and sensitive.
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A novel genomic map of the apogamous gametophyte of the fern Dryopteris affinis unlocks oldest hindrance with this complex plant group, to gain insight into evo-devo approaches. The gametophyte of the fern Dryopteris affinis ssp. affinis represents a good model to explore the molecular basis of vegetative and reproductive development, as well as stress responses.

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Article Synopsis
  • Pollinator-driven evolution of floral traits significantly influences the speciation and diversification of angiosperms, with Ophrys orchids employing sexual deception by mimicking female insects to attract male pollinators.
  • The study presents a comprehensive 5.2 Gb genome sequence of Ophrys sphegodes, revealing key genetic features such as transposable element expansion and gene duplication that aid in chemical mimicry and reproductive isolation.
  • A notably differentiated genomic region on chromosome 2 is linked to pollinator-mediated evolution, indicating that this genome can help explore the genetics behind repeated sexual deception and adaptations in pollinators.
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Shifts in pollinator occurrence and their pollen transport effectiveness drive the evolution of mating systems in flowering plants. Understanding the genomic basis of these changes is essential for predicting the persistence of a species under environmental changes. We investigated the genomic changes in Brassica rapa over nine generations of pollination by hoverflies associated with rapid morphological evolution toward the selfing syndrome.

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