Progress in the development of shrimp cell cultures in Thailand.

Primary shrimp cell cultures were developed from lymphoid organ and ovaries of black tiger shrimp, Penaeus monodon, in double-strength Leibovitz's L-15 medium supplemented with 15% fetal bovine serum, 1% glucose, 5 g/L NaCl, 15% shrimp meat extract. The optimum conditions for primary culture in vitro were obtained in L-15 medium with an osmolality of approximately 730 +/- 10 mmol/kg, a temperature range of 25--28 degrees C and incubation in a normal atmosphere. However, basal medium supplemented with 0.