Salmonella Typhimurium effector SseI regulates host peroxisomal dynamics to acquire lysosomal cholesterol.

Salmonella enterica serotype Typhimurium (Salmonella) resides and multiplies intracellularly in cholesterol-rich compartments called Salmonella-containing vacuoles (SCVs) with actin-rich tubular extensions known as Salmonella-induced filaments (SIFs). SCV maturation depends on host-derived cholesterol, but the transport mechanism of low-density lipoprotein (LDL)-derived cholesterol to SCVs remains unclear. Here we find that peroxisomes are recruited to SCVs and function as pro-bacterial organelle.

Predicting Leptospirosis Using Baseline Laboratory Tests and Geospatial Mapping of Acute Febrile Illness Cases Through Machine Learning-Based Algorithm.

Introduction Leptospirosis is a zoonotic infection caused by bacteria, which is reemerging in various regions and often poses a diagnostic challenge due to its nonspecific symptoms. While most infections are mild, severe cases occur in 5-10% of patients and are associated with high mortality, especially in areas with poor sanitation and urbanization. This study aims to investigate the association of specific parameters with leptospirosis diagnosis using a machine learning model and geographic mapping tools to identify spatial patterns and high-risk areas for the disease.

Clinical and high-resolution manometry of 801 patients with esophageal dysmotility, including achalasia, in relation to age.

Background And Aim: Spectrum of esophageal motility disorders may differ according to age, but studies on this are scanty, contradictory, and included small number of patients. Accordingly, we retrospectively analyzed data of a large sample of patients to study the spectrum of esophageal motility disorders in relation to age, and to evaluate the clinical profile and high-resolution manometry parameters and achalasia subtypes according to Chicago IV criteria.

Methods: Of 909 patients evaluated by high-resolution water perfusion or solid-state manometry during a 3-year period, data on 801 were finally analyzed.