Enhanced Electrochemiluminescence Detection of Dopamine Using Antifouling PEDOT-Modified SPEs for Complex Biological Samples.

Detecting medically important biomarkers in complex biological samples without prior treatment or extraction poses a major challenge in biomedical analysis. Electrochemical methods, specifically electrochemiluminescence (ECL), show potential due to their high sensitivity, minimal background noise, and straightforward operation. This study investigates the ECL performance of screen-printed electrodes (SPEs) modified with the conductive polymer poly(3,4-ethylenedioxythiophene) (PEDOT) and its derivatives for dopamine (DA) detection.

Chelation-Induced Zwitterion-like Antifouling Behavior on Anionic Poly(3,4-ethylenedioxythiophene) Surfaces.

Antifouling properties are crucial for enhancing the longevity and functionality of biomedical implants, drug delivery systems, and biosensors. Zwitterionic polymers are renowned for their exceptional surface hydration and charge neutrality, which effectively resist biomolecular adsorption and protein attachment. We propose an innovative approach to develop zwitterion-like antifouling surfaces by chelating divalent cations with anionic poly(3,4-ethylenedioxythiophene) (PEDOT) films, specifically PEDOT-PO and PEDOT-COOH.

Predictors of liver fibrosis changes assessed by paired liver biopsies in chronic hepatitis C patients treated with direct-acting antivirals.

Background/purpose: There are limited studies performing paired liver biopsies in chronic hepatitis C (CHC) patients treated with direct-acting antivirals (DAA). We aimed to investigate the predictors of liver fibrosis changes assessed by paired liver biopsies in these patients.

Methods: From March 2017 to March 2020, 113 CHC patients were prospectively enrolled to receive DAA therapy at our hospital.

Long-term surveillance of liver histological changes in chronic hepatitis C patients completing pegylated interferon-α plus ribavirin therapy: an observational cohort study.

Background: For chronic hepatitis C (CHC) patients completing pegylated interferon (PegIFN)-α/ribavirin therapy, long-term liver histological changes remain largely unexplored.

Methods: This observational cohort study included 85 CHC patients completing PegIFN-α/ribavirin therapy with liver biopsies performed at baseline and the end of surveillance (EOS). Median years between paired biopsies were 6.

Prognostic roles of diabetes mellitus and hypertension in advanced hepatocellular carcinoma treated with sorafenib.

Background & Aims: It remains limited whether diabetes mellitus (DM) and hypertension (HTN) affect the prognosis of advanced hepatocellular carcinoma (HCC) treated with sorafenib. Our study attempted to elucidate the roles of DM/HTN and the effects of diabetes medications among advanced HCC patients receiving sorafenib.

Methods: From August 2012 to February 2018, 733 advanced HCC patients receiving sorafenib were enrolled at China Medical University, Taichung, Taiwan.

Diacylglycerol lipase regulates lifespan and oxidative stress response by inversely modulating TOR signaling in Drosophila and C. elegans.

Target of rapamycin (TOR) signaling is a nutrient-sensing pathway controlling metabolism and lifespan. Although TOR signaling can be activated by a metabolite of diacylglycerol (DAG), phosphatidic acid (PA), the precise genetic mechanism through which DAG metabolism influences lifespan remains unknown. DAG is metabolized to either PA via the action of DAG kinase or 2-arachidonoyl-sn-glycerol by diacylglycerol lipase (DAGL).

Escherichia coli noncoding RNAs can affect gene expression and physiology of Caenorhabditis elegans.

Food and other environmental factors affect gene expression and behaviour of animals. Differences in bacterial food affect the behaviour and longevity of Caenorhabditis elegans. However, no research has been carried out to investigate whether bacteria could utilize endogenous RNAs to affect C.

Reduced expression of alpha-1,2-mannosidase I extends lifespan in Drosophila melanogaster and Caenorhabditis elegans.

Exposure to sub-lethal levels of stress, or hormesis, was a means to induce longevity. By screening for mutations that enhance resistance to multiple stresses, we identified multiple alleles of alpha-1,2-mannosidase I (mas1) which, in addition to promoting stress resistance, also extended longevity. Longevity enhancement is also observed when mas1 expression is reduced via RNA interference in both Drosophila melanogaster and Caenorhabditis elegans.