Metal chelate-epoxy bifunctional membranes for selective adsorption and covalent immobilization of a His-tagged protein.

The preparation and application of metal chelate-epoxy bifunctional membranes for the selective adsorption and covalent immobilization of His-tagged protein switch RG13 were shown in this study. By controlling the concentration of iminodiacetic acid (IDA) and reaction time during the conjugation of IDA on to the epichlorohydrin-activated regenerated cellulose membrane, 5 metal chelate-epoxy bifunctional membranes, with degrees of IDA conjugation in the range of 20%-81%, were prepared. The bifunctional membrane with an IDA conjugation degree of 30%, designated as BFM, exhibited a sound adsorption capacity of 0.