Protein Expression Analysis in Reversible Photobleached Cells of after High Temperature Stress.

We have analyzed protein expression in the bleached small vegetative cells of synchronous to investigate how unicellular algae lived through stress. These cells were subjected to heat treatment (46.5 °C for 1h in dark condition) and then cultured under continuous illumination for 24 h.

Metagenomic, phylogenetic, and functional characterization of predominant endolithic green sulfur bacteria in the coral Isopora palifera.

Background: Endolithic microbes in coral skeletons are known to be a nutrient source for the coral host. In addition to aerobic endolithic algae and Cyanobacteria, which are usually described in the various corals and form a green layer beneath coral tissues, the anaerobic photoautotrophic green sulfur bacteria (GSB) Prosthecochloris is dominant in the skeleton of Isopora palifera. However, due to inherent challenges in studying anaerobic microbes in coral skeleton, the reason for its niche preference and function are largely unknown.

Eliminating interference by anthocyanins when determining the porphyrin ratio of red plant leaves.

Anthocyanins (Ants) are water-soluble secondary metabolites that are responsible for red colour of plant leaves. To determine photosynthetic pigments, 80% acetone was used to extract Ants from Ant-containing leaves of test plants. However, using the 80% acetone extraction method can lead to interference between chlorophylls (Chls) and Ants.

The reversible degeneration of heat-treated Scenedesmus vacuolatus under continuous light cultivation conditions.

To find out how microalgae cope with heat stress, the small vegetative cells of a synchronous Scenedesmus vacuolatus culture were subjected to heat treatment and then cultured under continuous illumination. The heat-treated cells were found first to enter a degenerative intermediate stage with low cellular activities almost right after the start of the cultivation, which was then followed by a revival. The changes in physiological activities and morphology of the treated cells throughout the whole period of regeneration were explored.

Characterization of the decline and recovery of heat-treated Scenedesmus vacuolatus.

Background: To find out how algal cells cope with and recover from heat stress, the small vegetative cells of the synchronous Scenedesmus vacuolatus culture were subjected to a heat pretreatment (46.5°C for 1 h) followed by dark recultivation. The changes in physiological activities and morphology of Scenedesmus cells were continuously monitored throughout the course of decline and recovery.

DISINTEGRATION OF THE CELLS OF SIPHONOUS GREEN ALGA CODIUM EDULE (BRYOPSIDALES, CHLOROPHYTA) UNDER MILD HEAT STRESS(1).

The siphonous green alga Codium edule P. C. Silva (Bryopsidales, Chlorophyta) has the highest covering ratio among the macroalgae on the coral reef of Nanwan Bay in southern Taiwan, but its population in the subtidal region drastically decreases from July to September each year.