Interrelation Between Pathoadaptability Factors and Crispr-Element Patterns in the Genomes of Isolates Collected from Healthy Puerperant Women in Ural Region, Russia.

is a commensal and opportunistic bacterium widely distributed around the world in different niches including intestinal of humans and animals, and its extraordinary genome plasticity led to the emergence of pathogenic strains causing a wide range of diseases. is one of the monitored species in maternity hospitals, being the main etiological agent of urogenital infections, endometriosis, puerperal sepsis, and neonatal diseases. This study presents a comprehensive analysis of isolates obtained from the maternal birth canal of healthy puerperant women 3-4 days after labor.

Plain-nosed bats (family Vespertilionidae) as a possible reservoir of lyssaviruses and coronaviruses in Western Siberia and the south of European Russia.

The review presents current data on the chiropterofauna inhabiting Western Siberia and the south of the European part of Russia. A general description of the genus of lyssaviruses and the family of coronaviruses is given. The potential for virus carriage in relation to lyssaviruses and coronaviruses in bat populations of two geographically distant regions is considered.

CRISPR/Cas9 Landscape: Current State and Future Perspectives.

CRISPR (clustered regularly interspaced short palindromic repeats)/Cas9 is a unique genome editing tool that can be easily used in a wide range of applications, including functional genomics, transcriptomics, epigenetics, biotechnology, plant engineering, livestock breeding, gene therapy, diagnostics, and so on. This review is focused on the current CRISPR/Cas9 landscape, e.g.

Increasing the Thermal Stability and High-Temperature Strength of Vanadium Alloys by Strengthening with Nanosized Non-Metallic Particles.

Using the methods of scanning and transmission electron microscopy, the features of the structural-phase state of a vanadium alloy of the V-Cr-Ta-Zr system after a combined treatment, which consisted in cyclic alternation of thermomechanical and chemical-heat treatments, were studied. The values of yield strength and ductility of the V-Cr-Ta-Zr alloy were determined, depending on the stabilization and test temperatures. It was established that, after the combined treatment, the structural-phase state of the V-Cr-Ta-Zr alloy was composite, in which the joint implementation of dispersion and substructural strengthening ensured the formation of a gradient grain structure with a polygonal state, the elements of which were fixed by nanosized ZrO particles characterized by a high thermal stability.

Protocol for chronic hepatitis B virus infection mouse model development by patient-derived orthotopic xenografts.

Background: According to the World Health Organization, more than 250 million people worldwide are chronically infected with the hepatitis B virus, and almost 800.000 patients die annually of mediated liver disorders. Therefore, adequate biological test systems are needed that could fully simulate the course of chronic hepatitis B virus infection, including in patients with hepatocellular carcinoma.

Comparative Whole-Genome Analysis of Russian Foodborne Multidrug-Resistant Infantis Isolates.

Non-typhoidal infections remain a significant public health problem worldwide. In this study, we present the first detailed genomic analysis report based on short-read (Illumina) whole-genome sequencing (WGS) of 45 multidrug-resistant (MDR) subsp. serotype Infantis isolates from poultry and meat product samples obtained in Russia during 2018-2020, and long-read (MinION) WGS of five more representative isolates.

CRISPR Element Patterns vs. Pathoadaptability of Clinical Isolates from a Medical Center in Moscow, Russia.

is a member of the ESKAPE opportunistic pathogen group, which includes six species of the most dangerous microbes. This pathogen is characterized by the rapid acquisition of antimicrobial resistance, thus causing major healthcare concerns. This study presents a comprehensive analysis of clinical isolates based on whole-genome sequencing data.

Protocol for assessment of the efficiency of CRISPR/Cas RNP delivery to different types of target cells.

Background: Delivery of CRISPR/Cas RNPs to target cells still remains the biggest bottleneck to genome editing. Many efforts are made to develop efficient CRISPR/Cas RNP delivery methods that will not affect viability of target cell dramatically. Popular current methods and protocols of CRISPR/Cas RNP delivery include lipofection and electroporation, transduction by osmocytosis and reversible permeabilization and erythrocyte-based methods.

Genomic and Phenotypic Analysis of Multidrug-Resistant Clinical Isolates Carrying Different Types of CRISPR/Cas Systems.

is an opportunistic pathogen being one of the most important causative agents of a wide range of nosocomial infections associated with multidrug resistance and high mortality rate. This study presents a multiparametric and correlation analyses of clinical multidrug-resistant isolates using short- and long-read whole-genome sequencing, which allowed us to reveal specific characteristics of the isolates with different CRISPR/Cas systems. We also compared antibiotic resistance and virulence gene acquisition for the groups of the isolates having functional CRISPR/Cas systems, just CRISPR arrays without cas genes, and without detectable CRISPR spacers.

[EPIDEMIC SITUATION FOR HIV-INFECTION IN SIBERIAN FEDERAL REGION IN 2014].

Aim: Analyze main epidemiologic indicators for HIV-infection on the territory of SFR for the period from 2012 to 2014 with allotment of contributions of regions into the epidemic for development of personalized regional program for further containment of HIV spread.

Materials And Methods: Data of main statistical forms and monthly reports of all the regions situated on the territory of the district were used.

Results: At large, on both the studied territory and some regions a tendency of growth of HIV-infection morbidity with predominant parenteral transmission was detected.

[Indicators of the human immunodeficiency virus drug resistance to antiretroviral drugs in HIV-infected individuals in the Siberian Federal District in 2010-2012].

The prevalence of the mutations of resistance to the main three classes of antiretroviral agents in patients receiving antiretroviral therapy was tested. Among the main drug resistance mutations for the entire period of observation was a high frequency of the occurrence M184V mutation, K101E, K103N, Y181C, and G190S influencing the development of the HIV resistance to nucleoside and non-nucleoside reverse transcriptase inhibitors. The problems of the practical application of the study of HIV drug resistance in the regions of the Siberian Federal District were emphasized.

A study of the human bocavirus replicative genome structures.

The complete genomes of two human bocavirus 4 (HBoV4) isolates recovered in 2011 in Novosibirsk, Russia have been determined. A set of primers was designed based on the determined and previously published HBoV sequences; this primer pair was able to detect all possible HBoV replicative intermediates. This primer set was used to assay all HBoV genotypes and detected only those structures that correspond to an episomal form of this viral genome.

Recombination in the evolution of human bocavirus.

Whole genome sequencing of Novosibirsk human bocavirus (HBoV) isolates has detected an isolate that emerged via recombination between HBoV3 and HBoV4 genotypes. The recombination site is located between regions with abnormally low and abnormally high GC contents in the genome. This site is a bocavirus recombination hotspot and coincides with one of two parvovirus recombination hotspots.

Evolutionary time-scale of primate bocaviruses.

Human bocavirus (HBoV) is associated with acute gastroenteritis in humans, occurring mostly in young children and elderly people. Four bocavirus genotypes (HBoV1-HBoV4) have been found so far. Since there were no data on the contribution of HBoV to gastroenteritis in Russia, 1781 fecal samples collected from infants hospitalized with acute gastroenteritis in Novosibirsk, Russia during one year were tested for the presence of nucleic acids from HBoV and three major gastrointestinal viruses (rotavirus A, norovirus II, and astrovirus).