[FORMATION AND FUNCTIONING OF SYMBIOTIC SYSTEMS AND RHIZOSPHERE MICROBIOCENISIS OF SOYBEAN UNDER VARIOUS FUNGICIDES APPLICATION].

The Relevance. At the recent years in soybean crops the quantity of plant pathogenic fungi has increased. The fungicides of systemic and contact action have been applicated intensively against of them.

[INFLUENCE OF FUNGICIDES COMPLEX INOCULUM EKOVITAL ON RHIZOSPHERE MICROBIOCENOSIS, DISEASES RESISTANCE AND SOYBEEN PRODUCTIVITY].

It has been investigated the effect of fungicide with systemic action of Vitavaks 200 FF, Maxim Star 025 FS, Kinto duo and the complex inoculum Ekovital on rhizospheric microbial communities, diseases resistance and soybean productivity of Annushka biovar. The combined use of fungicides and inoculation has contributed to better formation of nodulation apparatus (the number of nodules on the roots was increased in 1.3-2.

Modification of (137)Cs transfer to rape (Brassica napus L.) phytomass under the influence of soil microorganisms.

After nuclear accidents, such as those experienced in Chernobyl and Fukushima, microorganisms may help purify contaminated soils by changing the mobility of radionuclides and their availability for plants by altering the physical and chemical properties of the substrate. Here, using model experiments with quartz sand as a substrate we investigate the influence of microorganisms on (137)Cs transfer from substrate to plants. The highest transition of (137)Cs from substrate to plants (50% increase compared to the control) was observed after Brassica napus L.

[Effect of soil microflora on 137Cs transition to plants].

The impact of certain types of microorganisms on 137Cs transfer from the substrate into the plant was analyzed in the experiment on artificial mediums. It was found that certain types of microorganisms could either reduce or increase the ratio of 137Cs transfer from the substrate to the plant. It is shown that this property is independent of the localization of the microorganism on the surface of the root, for all the analyzed bacteria belonging to the rhizospheric group.

[Comparative analysis of isolation methods of DNA from rhizosphere soil for research of microbiocenosis].

The comparative analysis of different methods for the isolation of microbial DNA from ordinary black earth and dark-gray podzolic soil of soybean rhizosphere was carried out. All preparations of DNA which isolated from dark-gray podzolic soil after purification using highly dispersed silica (AppliChem, Germany) were suitable for 16S rRNA genes amplification with universal bacterial primers 11F i 1492R. For DNA extraction from ordinary black earth suitable for PCR DNA preparations were obtained using two of the six investigated methods.

[Qualitative composition of dominating forms of microorganisms isolated from radionuclide contaminated soil and their ability to accumulate 137Cs].

Qualitative composition of the dominating forms of microorganisms isolated from radionuclide contaminated soils has been studied. The ability to accumulate 137Cs by freshly isolated species and collection cultures that were not adapted to the presence of the radionuclide has been analyzed. It is shown that among the analyzed microorganisms the greatest ability to accumulate the radionuclide is inherent in the collection culture Bacillus megaterium UKMV-5724.

[Activity of main enzymes of ammonium assimilation in Bradyrhizobium japonicum under the influence of plant flavonoid inductors].

The activity of key enzymes of ammonium assimilation in cell-free extracts of soybean rhizobia characterized with different effectiveness in symbiosis, has been researched. It has been found out that at highly efficient strain Bradyrhizobium japonicum UKM B-6035 the most active links in the system of ammonium assimilation reactions are glutamate synthase (glutamate-oxoglutarate-aminotransferase, GOGAT) and glutamate dehydrogenase (GDH). Whereas the most active links at the ineffective strain B.

[Effect of phytoregulator reglag on symbiotic properties of Bradyrhizobium japonicum 634b].

The influence of inoculation compositions on the basis of nodule bacteria Bradyrhizobium japonicum 634b and phytohormonal plant preparation Reglalg on formation, functioning and productivity of soybean-rhisobium symbiosis was studied in the vegetation and field experiments. It was shown, that the phytohormonal component of the composition increases the nodulation and nitrogenase activity of the Bradyrhizobium japonicum 634b and effectiveness of the symbiotic system.

[Glutamate dehydrogenase activity of Bradyrhizobium japonicum in the presence of phytoregulators].

Influence of plant growth regulators ivin and emistim C, and flavonoids daidzein and quercetin on the glutamate dehydrogenase activity of soybean nodule bacteria, with contrasting symbiotic properties, were studied. It was shown that all used phytoregulators stimulated glutamate dehydrogenase activity of Bradyrhizobium japonicum 71t (the strain with highly efficient symbiotic properties) 1.2-4.

[Effect of plant growth regulators on physiological activity of Bradyrhizobium japonicum ].

Influence of plant growth regulators Ivin, Emistim C, Eney and Agrostimulin on the biomass production and exopolymers synthesis of soybean nodule bacteria, which have contrasting symbiotic properties, and glutamine synthetase activity of their cell-free extracts were studied. It was shown that the processes of the biomass and exopolymers accumulation had an opposite direction. Of all preparations only Ivin and Agrostimulin intensificol growth activity of the microorganisms under study.