[Evaluation of the influence of exposure to tobacco smoke on the concentration of the pregnancy-associated plasma protein A in the population of healthy men and non-pregnant women].

The chemical variety of tobacco smoke components induces a multidirectional, not yet fully understood effects on living organisms, including both those actively and passively exposed. The chemical compounds present in tobacco smoke, due to its heterogeneous structure and properties undergo different biotransformation processes. Some components of tobacco smoke (for example, carbon monoxide) have an immediate effect on the body, in this case due to the formation of carboxyhemoglobin, and nicotine contained in the tobacco smoke also has direct influence on the cardiovascular system.

[Oral health in multiple sclerosis patients].

Multiple sclerosis (MS) is a chronic demyelinating disease of the central nervous system occuring in young adults, mainly female. MS dominates in Caucasians living in regions far away from the equator. The coexistence of genetic and environmental factors is considered in its etiopathogenesis.

Medicine students and exposure to environmental tobacco smoke.

Objective: Although medicine students express positive attitudes toward providing lifestyle counseling, they require more instruction in many areas of health behavior in order to be helpful to their patients. The presented study included the students' questionnaires analysis regarding their lifestyle and exposure to tobacco smoke. The aim of this study was to examine students' exposure to chosen xenobiotics by determination of selected biomarkers in urine samples, which underlay the basis for exposure assessment towards tobacco smoke.

[Assessment of exposure to tobacco smoke in a selected group of patients with multiple sclerosis from the Upper Silesia region].

In recent years, the increase in the incidence of multiple sclerosis (MS - Multiple Sclerosis) is observed. and the direct cause of the symptoms of multiple sclerosis is myelin nerves damage. It can be concluded that the environmental factor is at least partly responsible for the occurrence of this disease.

[3-nitrotyrosine determination as nitrosative stress marker and health attitudes of medical students considering exposure to environmental tobacco smoke].

Negative attitudes in health such as cigarette smoking and imbalanced diet play important role in pathogenesis of various diseases. Cigarette smoking constitutes one of the main sources of exposure to cancerogenic and procancerogenic xenobiotics among adults as well as among young people. Many studies have proven that cigarettes smokers more frequently follow less varied diet in comparison to non-smokers.

[Oxidative stress biomarkers determination in urine samples in the group of medical students exposed to tobacco smoke].

Cigarette smoking constitutes one of the main sources of exposure to cancerogenic and procancerogenic xenobiotics in the human population. Smoking is not only a bad habit among adults but also among young people. Despite many anti-tobacco campaigns undertaken by European Union countries still the smoking percentage among young people is high and does not decrease in the recent years.

The enzyme-linked immunosorbent assay (ELISA) method for nicotine metabolites determination in biological fluids.

The aim of this study was to present the usefulness of enzyme-linked immunosorbent assay (ELISA) in nicotine metabolites determination in urine and serum samples from active smokers and compare it with the reference to thin layer chromatography (TLC) with densitometry. The specific anti-cotinine antibodies were obtained from rabbit sera after sequential immunization with 4'-carboxycotinine-hemocyanine conjugate. The immunoaffinity chromatography technique with the use of self-prepared cotinine-aninohexyl-sepharose bed enabled the isolation of the specific anti-nicotine metabolites antibodies from the antiserum.

[The intranatal analysis of trans-3'-hydroxycotinine concentrations in serum of mother and neonate exposed to tobacco smoke].

The smoking habit is widely spread between pregnant women too. Tobacco smoke components have negative influence on infant's development. In human trans-3'-hydroxycotinine is besides cotinine, one of the main nicotine metabolites.

[Assessment of occurrence frequency of cotinine and trans-3'-hydroxycotinine in chosen body fluid samples].

Kind of the main nicotine metabolites, occurrence frequency of trans-3'-hydroxycotinine (HK) and cotinine (K) in mother and foetus serum, amniotic fluid and urine of chosen group of children (10 - 12 years old) determined by planar chromatography with densitometry as well as ratio of (HK)/(K) has been compared. The obtained data allowed to state, that the main nicotine metabolite concentrations (HK and K) were higher in foetus serum in comparison to mother serum. In the case of occurrence both of them ratio of (HK)/(K) was close to 1 in 20% of examined body fluid samples, but it was above this value in significant majority cases.

[Immunochemical methods in assessment of nicotine exposure].

This publication is the review of immunochemical methods applied in assessment of nicotine exposure. A review is coverage of nicotine metabolism, some aspects of specific antinicotine metabolites antibodies production, radioisotopic, immunoenzymatic and other techniques, as well as concerns their application in practice and further results interpretation.

[Chromatographic methods in tobacco smoke assessment].

In this paper review of chromatographic techniques and detectors used to analyse nicotine and its metabolites has been presented. The chromatographic determination of xenobiotics in biological material is usually proceeded by multistage isolation procedures, using liquid-liquid and liquid-solid extraction in various modifications. In our investigations, planar chromatography with densitometry has been used to identify and quantify nicotine and its metabolites in body fluids for assessment of tobacco smoke exposure.

Analysis of amylbenzene adsorption equilibria on different RP-HPLC.

For the purpose of description of the adsorption process of amylbenzene on a C8-, C18-, and C30-bonded silica stationary phase with methanol-water (80:20, v/v) as the mobile phase, a novel adsorption model (called the cluster isotherm model) is used. The model assumes the possibility of independent adsorption of analyte clusters on the longer C30 and shorter C8 chains. The validation of the proposed isotherm is made by comparison of experimental breakthrough and peak profiles obtained for RP-8e and RP-30 columns at a temperature of 24 degrees C and for RP-18e at a temperature range of 7-60 degrees C, with a theoretical simulation using the Transport-Dispersive model.