Publications by authors named "Tyler Stodden"
Article Synopsis
- Carfentanil ([C]CFN) is the only carbon-11 labeled radiotracer used for PET imaging of mu opioid receptors, but its effects in preclinical studies haven't been fully explored.
- In studies with anesthetized rats, researchers found that higher doses of CFN led to significant changes in vital signs and a correlation between CFN mass and mu opioid receptor availability in the brain.
- The results suggest that controlling CFN dosage is crucial to avoid complications and accurately measure mu opioid receptor activity, highlighting the need for careful quality control in PET studies with this radiotracer.
The continuous rise in opioid overdoses in the United States is predominantly driven by very potent synthetic opioids, mostly fentanyl and its derivatives (fentanyls). Although naloxone (NLX) has been shown to effectively reverse overdoses by conventional opioids, there may be a need for higher or repeated doses of NLX to revert overdoses from highly potent fentanyls. Here, we used positron emission tomography (PET) to assess NLX's dose-dependence on both its rate of displacement of [C]carfentanil ([C]CFN) binding and its duration of mu opioid receptor (MOR) occupancy in the male rat brain.
View Article and Find Full Text PDFExcessive alcohol consumption is associated with neuroinflammation, which likely contributes to alcohol-related pathology. However, positron emission tomography (PET) studies using radioligands for the 18-kDa translocator protein (TSPO), which is considered a biomarker of neuroinflammation, reported decreased binding in alcohol use disorder (AUD) participants compared to controls. In contrast, autoradiographic findings in alcohol exposed rats reported increases in TSPO radioligand binding.
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- The central adenosine A receptor (AR) is important in various health issues, including pain, sleep disorders, and neurodegenerative diseases, making it a key target for drug development.
- Current AR PET radiotracers primarily use xanthine-based antagonists, which have not effectively outcompeted endogenous adenosine.
- The study introduces a new, non-nucleoside PET radiotracer (MMPD, 22b) that shows high affinity for AR, good brain permeability, and the ability to detect changes in endogenous adenosine levels.
Neuroinflammation appears to contribute to neurotoxicity observed with heavy alcohol consumption. To assess whether chronic alcohol results in neuroinflammation we used PET and [C]PBR28, a ligand that binds to the 18-kDa translocator protein (TSPO), to compare participants with an alcohol use disorder (AUD: n = 19) with healthy controls (HC: n = 17), and alcohol-dependent (n = 9) with -nondependent rats (n = 10). Because TSPO is implicated in cholesterol's transport for steroidogenesis, we investigated whether plasma cholesterol levels influenced [C]PBR28 binding.
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