Fabrication of herbicide biosensors based on the inhibition of enzyme activity that catalyzes the scavenging of hydrogen peroxide in a thylakoid membrane.

A novel herbicide biosensor with a thylakoid modified membrane electrode is presented. Thylakoid, isolated from spinach leaves, was entrapped in a membrane of poly (vinylalcohol) with the styrylpyridinium group (PVA-SbQ). The thylakoid membrane was fixed on the surface of a platinum electrode.

Charge-selective recognition at fibroin-modified electrodes for analytical application.

A novel fibroin-modified electrode with charge recognition is reported. The characteristics of silk fibroin membranes have been exploited for analytical applications. The membrane, with an isoelectric point of pH 4.

Electrocatalytic oxidation of hydrogen peroxide and cysteine at a glassy carbon electrode modified with platinum nanoparticle-deposited carbon nanotubes.

A glassy carbon electrode modified with platinum nanoparticle-decorated carbon nanotubes (Pt-CNT/GCE) was prepared. The electrochemical behaviors for the catalysis oxidations of hydrogen peroxide and cysteine were studied. The Pt-CNT/GCE showed catalytic activity for electro-oxidation of hydrogen peroxide at 0.

Electrochemical determination of interaction parameters for DNA and mitoxantrone in an irreversible redox process.

Mitoxantrone (MXT), an anti-tumor antibiotic, shows irreversible electrochemical behavior at a waxed graphite electrode in a 0.05 M Tris-HCl buffer (pH 7.4) solution.

Investigation on the interaction of DNA and electroactive ligands using a rapid electrochemical method.

A rapid method for investigation of the interaction of DNA and electroactive ligands based on an electrochemical equation for irreversible processes is presented. The binding constant (K) and the size of binding site (s) are simultaneously obtained from the dependence of the current on the amount of added DNA in voltammetry. A non-intercalative binder (Hoechst 33258) and two DNA-intercalators (mitoxantrone (MXT) and actinomycin D (AMD)) were examined in experiments.