Publications by authors named "Tushar Kashinath Manape"
Article Synopsis
- CRISPR/Cas9 is a precise genome editing tool that has yet to be explored in onions, making this study significant as it reports the first successful use of this technique in the species.
- The research targeted the Phytoene desaturase gene in onion cv. Bhima Super using a specific transformation protocol, resulting in 21 regenerated shoots with noticeable phenotypic variations such as albino, chimeric, and pale green plants.
- Analysis revealed mutations leading to the albino phenotype, confirming effective gene editing, and highlighting potential avenues for future research on genetically modified onions.
Cuticular wax is a characteristic feature of land plants that provides protection against both biotic and abiotic stresses. In this study, a glossy mutant lacking an epicuticular wax layer was identified in the γ-irradiated M mutant population of the onion cultivar Bhima Super. The inheritance of the mutant's glossy phenotype was determined to be recessive and single locus.
View Article and Find Full Text PDFBackground: MSH1 (MutS homolog1) is a nuclear-encoded protein that plays a crucial role in maintaining low mutation rates and stability of the organellar genome. While plastid MSH1 maintains nuclear epigenome plasticity and affects plant development patterns, mitochondrial MSH1 suppresses illegitimate recombination within the mitochondrial genome, affects mitochondrial genome substoichiometric shifting activity and induces cytoplasmic male sterility (CMS) in crops. However, a detailed functional investigation of onion MSH1 has yet to be achieved.
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