Three new monoterpenes compounds isolated from Seriphidium terrae-albae exerted anti-inflammatory effects through the JAK/STAT and NF-κB signaling pathways.

Three new monoterpenes compounds (5S, 8S)-5-(2E-butenyl)-8-methyl propionate-cyclopentanone (1), 1-Oxy, 10-keto-α-myrcene hydroxide (2), (3R,4R)-3-hydroxy-4-isobutenyl-cyclopentyl ester (3), along with eleven known small molecular compounds such as monoterpenes (1-7, 14), coumarin (10), and other small molecular compounds (8, 9, 11-13) were isolated from Seriphidium terrae-albae. The structures were elucidated by NMR, HRESIMS, ECD calculations, and X-ray crystallography. Anti-inflammatory activity test results showed that 9 compounds were detected to inhibit NO secretion by mouse macrophage Raw 264.

Sesquiterpenes from the seeds of Cichorium glandulosum and their anti- neuroinflammation activities.

Three previously undescribed sesquiterpenes, along with three known ones were isolated from the seeds of Cichorium glandulosum. The structures of them were elucidated by the analysis of spectroscopic data. The isolated compounds were tested for their neuroprotective effects against LPS-induced neuroinflammation in BV-2 cells.

Guaianolide sesquiterpene lactones from Cichorium glandulosum and their anti-neuroinflammation activities.

Eight undescribed guaianolide sesquiterpene lactones cicholosumins A-H and twelve known ones were isolated from the aerial parts of Cichorium glandulosum Boiss et Huet. Their structures were established by 1D and 2D NMR spectroscopic data, electronic circular dichroism, quantum chemical calculations and single crystal X-ray diffraction analysis. Compounds 9α-hydroxy-3-deoxyzaluzanin C, epi-8α-angeloyloxycichoralexin, 8-O-methylsenecioylaustricin and lactucin showed strong anti-neuroinflammation activity with IC values of 1.

New Phenylpropanoids and Disulphides Dimers from Ferula Kuhistanica.

Five undescribed compounds, including three phenylpropanoid derivatives, 4'-methoxycinnamyl isobutyrate (1), 4'-methoxycinnamyl-2"-methyl butyrate (2) and (2Z)-3',4'-dimethoxycinnamyl isovalerate (3) and two disulphides dimers, kuhistanicasulphide A (7) and kuhistanicasulphide B (8) together with five known ones, including three phenylpropanoids (4-6) and two disulphides (9-10), were isolated from the roots of Ferula kuhistanica Korovin. Their structures were elucidated on the basis of spectroscopic analysis, including IR, UV, HRESIMS, NMR and quantum C NMR DP4+ probability. Anti-inflammatory and cytotoxic (Hela, A549 and HT-29 cell lines) activities of the obtained compounds was tested, which compounds 4 and 5 demonstrated good anti-inflammatory with IC values of 25.

Unveiling the anti-vitiligo, anti-inflammatory, and antitumor activities of sesquiterpene coumarins isolated from Ferula kuhistanica.

Six undescribed bicyclic sesquiterpene coumarins, kuhistanin A, ferukrin isovalerate, 9'β,12'α - ferukrin isovalerate, (17'E)- 9'α, 12'β - isomarcandin, (17'Z)- 9'α, 12'β - isomarcandin and (17'E) - isomarcandin, together with nine known ones were isolated from the roots of Ferula kuhistanica Korovin. The structures of them were elucidated using NMR and HRESIMS data analysis. The relative configurations of the isolates were confirmed by NOE correlations and NMR calculation.

Spectrum-effect relationship between ultra-performance liquid chromatography fingerprints and melanogenic effect of Vernonia anthelmintica effective part.

The fingerprint of Vernonia anthelmintica effective part (VAEP) from 15 different producing areas was established, followed by cluster analysis and principal component analysis. The relationship between the fingerprint and the melanogenesis-promoting activity of VAEP was then analyzed using the grey correlation degree and the orthogonal partial least square method. The characteristic peaks reflecting the pharmacodynamic effect of VAEP were identified as vernodalin, 3,5-O-dicaffeoyl quinic acid (3,5-diCQA), and butin.

Chemical Composition of Artemisia Scoparia and Their Bioactivities.

Three undescribed sesquiterpenes (1-3), two enantiomeric pairs of monoterpenes (4a/4b-5a/5b), one alkyne (6), two known alkynes (7-8) and eight known coumarins (9-16) were isolated from the aerial parts extracts of Artemisia scoparia. The structures of these compounds were fully elucidated by their 1D and 2D NMR, HRESIMS spectral data analyses, and comparison with literature. The absolute configurations of compounds were determined by single-crystal X-ray crystallography (1), a comparison of experimental and calculated electronic circular dichroism (ECD) data (2-6).

Guaianolide sesquiterpenes and their activity from Artemisia mongolica.

Seven undescribed sesquiterpenes, including three dimeric guaianolide sesquiterpenes artemongolides G-I (1-3) and four sesquiterpene lactones artemanomalide D-G (16-19), along with seventeen known compounds isoabsinthin (4), absinthin (5), 11-eptabsinthin (6), 11, 11'-bis-epiabsinthin (7), 10', 11'- epiabsinthin (8), anabsinthin (9), isoanabsinthin (10), absinthin D (11), anabsin (12), caruifolin D (13), gnapholide (14), caruifolin C (15), 1β(R),10β(S)-dihydroxy-3-oxo-11β (S)H-4,11(13)-guaien-6α(S),12-olide (20), 1α,6α,8α-trihydroxy-5α,7βH-guaia-3,10(14),11(13)-trien-12-oic acid (21), 1α,6α,8α-trihydroxy-5α,7βH-guaia-3,9,11(13)-trien-12-oic acid (22), argyinolide J (23), artabsinolide A (24) were isolated from the plant Artemisia mongolica. The structures were determined by interpreting NMR, HRESIMS and ECD data. The X-ray crystal structure of 4, 7 and 8 were reported for the first time.

Diprenylated phenolic enantiomers from Artemisia scoparia.

Investigation on the chemical constituents of Artemisia scoparia resulted in the isolation of sixteen compounds, including undescribed six pairs of diprenylated phenolic enantiomers (±)-scopacoumaricin A-F, and two pairs of cis-trans isomers cis/trans-scopacoumaricin G and cis/trans-artepillin A. Trans-artepillin A was obtained from this plant for the first time. The structures of the isolates were proposed by analysis of their 1D, 2D-NMR and HRESIMS spectroscopic data.

Two novel sesquiterpenes and one new monoterpene from the aerial part of .

Two novel sesquiterpenes and one new monoterpene, together with eight reported compounds were isolated from dichloromethane-soluble extract of the aerial part of Reichb. Their relative and absolute structures were elucidated based on the analysis of 1D and 2D NMR spectra, HRESIMS, and calculated electronic circular dichroism (ECD). Two sesquiterpenes ( and ) showed no inhibition effect in anti-inflammatory and cytotoxic activity tests.

Sesquiterpenoids from and Their Melanogenic Activity.

A sesquiterpenoid with an unprecedented 5/5/4 tricyclic skeleton (), a -sesquiterpenoid with a rare 6/7 bicyclic skeleton (), 10 new sesquiterpenoids (-), and six known analogues (-) were isolated from the whole plants of . The structures of compounds - were elucidated by spectroscopic data analysis. Compound showed melanogenic promotion activity in murine melanoma (B16) cells more potent than the positive control used, 8-methoxypsoralen (8-MOP).

Oxygen heterocyclic Diels-Alder-Type sesquiterpenoid dimers from Vernonia anthelmintica.

Eleven undescribed oxygen heterocyclic Diels-Alder-Type sesquiterpenoid dimers, vernodalidimer I-K and M-T, along with a known one, vernodalidimer B were isolated from the seeds of Vernonia anthelmintica. The structures of them were elucidated based on 1D, 2D-NMR experiments and HRESIMS. The absolute configurations of vernodalidimer I-K and M-T were determined by comparison of their experimental and calculated electronic circular dichroism spectra.

Megastigmane sesquiterpenoids from whole plants of Viola kunawurensis.

Investigation on the chemical constituents of Viola kunawurensis resulted in the isolation of seven undescribed megastigmane sesquiterpenoids including four bicyclic megastigmane glucosides, kunawuronoside A-D, two megastigmane glucosides, kunawuronoside E-F, and a megastigmane, kunawurone A, together with ten known megastigmane sesquiterpenoids. Their structures were established by comprehensive 1D, 2D-NMR and HRESIMS analyses, and their absolute configurations were determined by comparing their calculated ECD data with the experimental ones. Evaluations of the anti-inflammatory activity revealed that kunawuronoside A-D and compounds 14-15 inhibited COX-2 expression with inhibition rates ranging from 36.

Sesquiterpene lactones from Artemisia mongolica.

Twelve undescribed sesquiterpene lactones and seven known sesquiterpene lactones were isolated from the whole plant of Artemisia mongolica. Their structures were elucidated based on the interpretation of NMR, HRESIMS, and electronic circular dichroism (ECD) data. The absolute configurations of mongolicolide A (1), mongolicolide C (3), 1R, 3S-Dihydroxy-7S, 11R-germacra-4Z.

Sesquiterpenes from Artemisia Sieversiana and their anti-inflammatory activities.

Eight undescribed sesquiterpenes, including three dimeric guaianolides and five sesquiterpens, together with ten known ones, were isolated from the aerial parts of Artemisia sieversiana. Their structures were elucidated by extensive spectroscopic techniques and ECD calculation. The isolated compounds were tested for their anti-inflammatory effects against IL-1β, IL-6 and TNF-α in LPS-induced RAW 264.

Quantitative Determination of Marker Compounds and Fingerprint Analysis of the Seeds of .

In traditional Chinese medicine, the seeds of (L.) Willd. have been widely used for treatment of cough, skin diseases, diarrhea, fever, schistosomiasis, amoebic dysentery, and gastrointestinal problems, especially in the treatment of vitiligo for thousands of years in China.

Secondary metabolites produced by endophytic derived from roots of and their effect on melanin synthesis in murine B16 cells.

Five indole derivatives, 1-indol-7-ol (), tryptophol (), 3-indolepropionic acid (), tryptophan (), 3,3-di(1-indol-3-yl)propane-1,2-diol () and two diketopiperazines, cyclo(L-Pro-L-Tyr) (), cyclo[L-(4-hydroxyprolinyl)-L-leucine () along with one dihydrocinnamic acid () were isolated from VERA8, that endophytic bacteria derived from roots. This is a first report towards an isolation of endophytic strains (funji or bacteria) from the herb. The synergetic properties of the total extract compositions, as well as effects of the pure isolated secondary metabolites evaluated on their melanin synthesis in murine B16 cells towards for vitiligo treatment.

Three new elemanolides from the seeds of Vernonia anthelmintica.

Three new elemanolides, named vernonilides D (1), E (2), and F (3), along with four known sesquiterpenoids, including two elemanolides (4, 5), a guaianolide (6), and a germacranolide (7) were isolated from the seeds of Vernonia anthelmintica. The structures of them were elucidated based on 1D and 2D NMR experiments and comparison with published data. Cytotoxicity of the compounds against four human tumor cell lines was assayed.

Optimization of Extraction Process for Antidiabetic and Antioxidant Activities of Kursi Wufarikun Ziyabit Using Response Surface Methodology and Quantitative Analysis of Main Components.

By using extraction yield, total polyphenolic content, antidiabetic activities (PTP-1B and -glycosidase), and antioxidant activity (ABTS and DPPH) as indicated markers, the extraction conditions of the prescription Kursi Wufarikun Ziyabit (KWZ) were optimized by response surface methodology (RSM). Independent variables were ethanol concentration, extraction temperature, solid-to-solvent ratio, and extraction time. The result of RSM analysis showed that the four variables investigated have a significant effect ( < 0.

Two new compounds from the seeds of Vernonia anthelmintica.

Two new compounds, named benzoyl-vernovan (1) and 2-(4'-hydroxyphenyl)-6-methyl-4H-pyran-4-one (2), together with one biflavonoid (3), one aurone (4) and six flavonoids were isolated from the seeds of Vernonia anthelmintica. Their structures were elucidated by extensive spectroscopic analyses and comparison with published data, and their influence on melanin content in B16 melanoma cells were tested. 5 and 9 increased melanin content by 2.

Elemanolide dimers from seeds of Vernonia anthelmintica.

The seeds of Vernonia anthelmintica afforded three new elemanolide dimers, vernodalidimers C, D, and E, and four known elemanolides. Their structures were elucidated based on extensive spectroscopic analysis (IR, HR-ESIMS, 1D- and 2D-NMR), and the absolute configurations of the new dimers were determined by comparison of the experimental and calculated electronic circular dichroism spectra. Cytotoxicity of the isolated compounds against four human tumor cell lines was assayed.

Dimeric guaianolides from Artemisia absinthium.

Five dimeric guaianolides, absinthins A-E, and seven known dimeric guaianolides were isolated from Artemisia absinthium. Their structures were elucidated based on 1D- and 2D-NMR experiments, including (1)H NMR, (13)C NMR, DEPT, (1)H-(1)H COSY, HSQC, HMBC, and NOESY, and through HRESIMS data analysis. The absolute configuration of the known compound, anabsinthin, was determined by X-ray crystallographic analysis.