Publications by authors named "Tuomas Mansikkala"
Salla disease (SD) is a lysosomal storage disease where free sialic acid (SA) accumulates in lysosomes due to the impaired function of a membrane protein, sialin. Synchrotron radiation-based scanning transmission soft X-ray spectromicroscopy (STXM) was used to analyze both SD patients' fibroblasts and normal human dermal fibroblasts (NHDF) from healthy controls. Both cell lines were also cultured with -acetyl-d-mannosamine monohydrate (ManNAc) to see if it increased SA concentration in the cells.
View Article and Find Full Text PDFArticle Synopsis
- Lignans, bioactive compounds prevalent in Norway spruce knotwood, were analyzed for their quantity and localization using advanced techniques such as chromatography and microscopy, revealing 15 unique lignans that accounted for 16% of the knotwood's dry weight and 82% of acetone extract.* -
- The primary lignans identified were hydroxymatairesinols HMR1 and HMR2, with various methods demonstrating their uneven distribution within cell lumina in the wood tissue.* -
- The study compared results from scanning transmission X-ray microscopy (STXM) and gas chromatography, confirming the composition of structural components like lignin and polysaccharides, while suggesting aromatic compounds, possibly lignin, were found in bordered pit
Human-induced pluripotent stem cell-derived cardiomyocytes (hiPSC-CMs) have the potential to serve as a model for human cardiomyocytes. However, hiPSC-CMs are still considered immature. CMs differentiated from hiPSCs more resemble fetal than adult cardiomyocytes.
View Article and Find Full Text PDF