CRISPR Based Programmable RNA Editing in Primary Hippocampal Neurons.

Investigating the RNA regulation landscape primarily relies on understanding how RNA-protein interactions are governed in various cell types, including neurons. Analysis of RNA-protein interactions in physiological environments warrants the development of new tools that rely on RNA manipulation. Recently, a CRISPR-based RNA-editing tool (dCas13b-ADAR2 ) was developed to mitigate disease-associated point mutations in cell lines.