Publications by authors named "Tuifan Yu"
Article Synopsis
- Trichoderma longibrachiatum UN32 is a significant fungus known for producing Dendrobine-Type Total alkaloids (DTTAs), with branched-chain amino acid aminotransferases (BCATs) playing a key role in this process.
- Research indicates that cobalt chloride can boost DTTAs production by enhancing the expression of the BCAT gene, TlBCAT, which was successfully cloned and characterized.
- TlBCAT can efficiently catalyze reactions involving branched-chain amino acids and exhibits broad substrate specificity, suggesting that adding exogenous BCAAs may further promote both the fungus's growth and DTTAs production.
Article Synopsis
- - The mutant strain Trichoderma longibrachiatum UN32 is known for producing a type of alkaloid called dendrobine-type total alkaloids (DTTAs), and adding cobalt (Co) to its growth medium enhances this production while affecting antioxidase-related genes.
- - Research showed that cobalt-induced reactive oxygen species (ROS), specifically hydrogen peroxide, positively impacts DTTAs biosynthesis; cobalt chloride (CoCl) significantly increased DTTAs production compared to other treatments.
- - However, when ROS scavengers like vitamin C and melatonin were added, ROS levels decreased significantly, leading to a reduction in DTTAs production, indicating that a specific range of ROS is essential for optimal fermentation and
species, which are perennial herbs widely distributed in tropical and subtropical regions, are notable for their therapeutic properties attributed to various bioactive compounds, including dendrobine-type sesquiterpenoid alkaloids (DTSAs). The objective of this review article is to provide a comprehensive overview of recent advances in the biosynthesis of DTSAs, including their extraction from species and endophytes, elucidation of associated genes through genomic and transcriptomic sequencing in both spp. and endophytes, exploration of the biosynthetic pathways of DTSAs, and drawing conclusions and outlining future perspectives in this field.
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