Is cervical swab an efficient method for developing a new noninvasive prenatal diagnostic test for numerical and structural chromosome anomalies?

Background/aim: Prenatal diagnosis is vital to obtain healthy generation for risky pregnancies. There have been several approaches, some of which are routinely applied in clinics to evaluate the possible prenatal deficiencies and/or diseases. In the present study, we aimed to isolate the fetal cells from endocervical samples and try to identify possible anomalies which were proved by Amniocentesis (AS) and chorionic villus sampling (CVS) methods.

Aflatoxin-specific monoclonal antibody selection for immunoaffinity column development.

Antibodies are the basic components of immunoanalytical systems used for detection of a wide range of analytes. Although there are some ground rules for antibody selection, analyte- and assay-specific criteria are the ones that determine the ultimate success of the immunoassays. In this study, we introduced an effective antibody selection procedure for the development of immunoaffinity columns for aflatoxins.

Determination of Aflatoxins and Ochratoxin A in Traditional Turkish Cereal-Based Fermented Food by Multi-Affinity Column Cleanup and LC Fluorescence Detection: Single-Laboratory Validation.

Tarhana is a traditional fermented, sun-dried Turkish food containing yogurtand cereals. There are several potential sources of mycotoxins in tarhana, such as contamination of ingredients or formation during preparation, when water activity is suitable for fungal growth and may lead to mycotoxin production during fermentation or subsequent sun-drying. To optimize an immunoaffinity column method and carry out single-laboratory validation for the determination of aflatoxins B, B₂, G, and G₂ together with ochratoxin A (OTA) in tarhana.

Determination of Aflatoxins and Ochratoxin A in Traditional Turkish Concentrated Fruit Juice Products by Multi-Immunoaffinity Column Cleanup and LC Fluorescence Detection: Single-Laboratory Validation.

: Pekmez and pestil are traditional Turkish foods made from concentrated grape juice, which can be contaminated with mycotoxins such as aflatoxins and ochratoxin A (OTA). : To carry out a single-laboratory validation of a method to simultaneously determine aflatoxins B, B₂, G, and G₂ and ochratoxin A in pekmez and pestil. : The homogenized sample is extracted with methanol-water (80 + 20) using a high-speed blender.