Genetic polymorphisms of FSHR, CYP17, CYP1A1, CAPN10, INSR, SERPINE1 genes in adolescent girls with polycystic ovary syndrome.

Background: Polycystic ovary syndrome (PCOS), whose genetic basis is not completely well understood, is the most common endocrine disorder in women and it typically develops during adolescence. The aim of this study is to investigate the possible association between single nucleotide polymorphisms (SNPs) of FSHR, CYP17, CYP1A1, CAPN10, INSR, SERPINE1 genes and PCOS in adolescent girls.

Methods: DNA samples from forty-four adolescent girls with PCOS and 50 healthy controls were analyzed by PCR-RFLP and direct DNA sequencing to determine the genotypic frequency of 17 different polymorphic loci on the FSHR (A307T, N680S), CYP17 (-34 T/C), CYP1A1 (T6235C), CAPN10 (44, 43, 19, 63), INSR (exon 17 C/T), SERPINE1 (4G/5G) genes.

[Allogeneic blood transfusion policy in orthopedic surgery].

Objectives: This retrospective study was performed to evaluate transfusions in orthopedic surgery and, in the light of our successful practice, to contribute to the development of appropriate transfusion policies in Turkey.

Methods: In this study 1,811 female patients who underwent orthopedic elective or emergency surgery from January 1997 to December 1999 (group 1) and from January 2000 to December 2002 (group 2) were retrospectively evaluated with respect to ordered and transfused blood units. Autologous blood transfusions were not included.