Complete genome sequence of ST462 harboring the gene on the chromosome, isolated from a pediatric diarrhea patient in Southern Vietnam.

Colistin resistance threatens global health as it compromises the effectiveness of a last-resort antibiotic. We present the complete genome sequence of ST462, which carries the gene, isolated from a pediatric diarrhea case in southern Vietnam. The 5,049,362 bp genome contains 24 resistance genes distributed across 107 contigs.

[How I treat… An open bite secondary to ankylosis of a primary molar tooth].

Infraclusion is a consequence of dentoalveolar ankylosis. Associated complications include extrusion of the opposing tooth, open bites, and tilts. Early detection and management are crucial to limit and/or correct these complications.

The potential of baicalin to enhance neuroprotection and mitochondrial function in a human neuronal cell model.

Baicalin is a flavone glycoside derived from flowering plants belonging to the Scutellaria genus. Previous studies have reported baicalin's anti-inflammatory and neuroprotective properties in rodent models, indicating the potential of baicalin in neuropsychiatric disorders where alterations in numerous processes are observed. However, the extent of baicalin's therapeutic effects remains undetermined in a human cell model, more specifically, neuronal cells to mimic the brain environment in vitro.

Network-based drug repurposing for schizophrenia.

Despite recent progress, the challenges in drug discovery for schizophrenia persist. However, computational drug repurposing has gained popularity as it leverages the wealth of expanding biomedical databases. Network analyses provide a comprehensive understanding of transcription factor (TF) regulatory effects through gene regulatory networks, which capture the interactions between TFs and target genes by integrating various lines of evidence.

Use of gene regulatory network analysis to repurpose drugs to treat bipolar disorder.

Background: Bipolar disorder (BD) presents significant challenges in drug discovery, necessitating alternative approaches. Drug repurposing, leveraging computational techniques and expanding biomedical data, holds promise for identifying novel treatment strategies.

Methods: This study utilized gene regulatory networks (GRNs) to identify significant regulatory changes in BD, using network-based signatures for drug repurposing.

Signaling pathways involved in microbial indoor air pollutant 3-methyl-1-butanol in the induction of stomatal closure in Arabidopsis.

Indoor air pollution is a global problem and one of the main stress factors that has negative effects on plant and human health. 3-methyl-1-butanol (3MB), an indoor air pollutant, is a microbial volatile organic compound (mVOC) commonly found in damp indoor dwellings. In this study, we reported that 1 mg/L of 3MB can elicit a significant reduction in the stomatal aperture ratio in Arabidopsis and tobacco.

Effects of antipsychotic drugs on energy metabolism.

Schizophrenia (SCZ) is a complex neuropsychiatric disorder associated with altered bioenergetic pathways and mitochondrial dysfunction. Antipsychotic medications, both first and second-generation, are commonly prescribed to manage SCZ symptoms, but their direct impact on mitochondrial function remains poorly understood. In this study, we investigated the effects of commonly prescribed antipsychotics on bioenergetic pathways in cultured neurons.

Uncovering molecular mechanisms involved in microbial volatile compounds-induced stomatal closure in Arabidopsis thaliana.

Microbial volatile compounds (mVCs) may cause stomatal closure to limit pathogen invasion as part of plant innate immune response. However, the mechanisms of mVC-induced stomatal closure remain unclear. In this study, we co-cultured Enterobacter aerogenes with Arabidopsis (Arabidopsis thaliana) seedlings without direct contact to initiate stomatal closure.

Volatile Compounds Emitted by Plant Growth-Promoting Fungus Tolypocladium inflatum GT22 Alleviate Copper and Pathogen Stress.

Previous studies on the intricate interactions between plants and microorganisms have revealed that fungal volatile compounds (VCs) can affect plant growth and development. However, the precise mechanisms underlying these actions remain to be delineated. In this study, we discovered that VCs from the soilborne fungus Tolypocladium inflatum GT22 enhance the growth of Arabidopsis.

N-terminal LysSN-His-tag improves the production of intracellular recombinant protein in Bacillus subtilis.

Choosing fusion tags to enhance the recombinant protein levels in the cytoplasm of Bacillus subtilis has been limited. Our previous study demonstrated that His-tag at the N-terminus could increase the expression levels of the low-expression gene egfp, while significantly reducing the high-expression genes gfp+ and bgaB in the cytoplasm of B. subtilis.

Potent IPTG-inducible integrative expression vectors for production of recombinant proteins in Bacillus subtilis.

The IPTG-inducible promoter family, Pgrac, allows high protein expression levels in an inducible manner. In this study, we constructed IPTG-inducible expression vectors containing strong Pgrac promoters that allow integration of the transgene at either the amyE or lacA locus or both loci in Bacillus subtilis. Our novel integrative expression vectors based on Pgrac promoters could control the repression of protein production in the absence and the induction in the presence of an inducer, IPTG.

Synthesis and Characterization of Novel Core-Shell ZnO@SiO Nanoparticles and Application in Antibiotic and Bacteria Removal.

A novel core-shell nanomaterial, ZnO@SiO, based on rice husk for antibiotic and bacteria removal, was successfully fabricated. The ZnO@SiO nanoparticles were characterized by X-ray diffraction (XRD), energy-dispersive X-ray spectroscopy (EDX), Fourier transform infrared (FTIR) spectroscopy, transmission electron microscopy (TEM), photoluminescence spectroscopy (PL), Brunauer-Emmett-Teller (BET) method, diffuse reflectance ultraviolet-vis (DR-UV-vis) spectroscopy, X-ray photoelectron spectroscopy (XPS), Raman spectroscopy, and ζ-potential measurements. β-Lactam antibiotic amoxicillin (AMX) was removed using ZnO@SiO nanoparticles with an efficiency greater than 90%, while removal was higher than 91%.

Studying the characteristics of nanobody CDR regions based on sequence analysis in combination with 3D structures.

Background: Single-domain antibodies or nanobodies have recently attracted much attention in research and applications because of their great potential and advantage over conventional antibodies. However, isolation of candidate nanobodies in the lab has been costly and time-consuming. Screening of leading nanobody candidates through synthetic libraries is a promising alternative, but it requires prior knowledge to control the diversity of the complementarity-determining regions (CDRs) while still maintaining functionality.

Metformin is Protective Against the Development of Mood Disorders.

Introduction: Mood disorders are a major cause of disability, and current treatment options are inadequate for reducing the burden on a global scale. The aim of this project was to identify drugs suitable for repurposing to treat mood disorders.

Methods: This mixed-method study utilized gene expression signature technology and pharmacoepidemiology to investigate drugs that may be suitable for repurposing to treat mood disorders.

Influence of N-terminal His-tags on the production of recombinant proteins in the cytoplasm of .

The influence of fusion tags to produce recombinant proteins in the cytoplasm of is not well-studied as in . This study aimed to investigate the influence of His-tags with different codons on the protein production levels of the high expression gene () and low expression gene () in the cytoplasm of cells. We used three different N-terminal His-tags, M-6xHis, MRGS-8xHis and MEA-8xHis, to investigate their effects on the production levels of GFP variants under the control of the P212 in .

Repurposing Drugs via Network Analysis: Opportunities for Psychiatric Disorders.

Despite advances in pharmacology and neuroscience, the path to new medications for psychiatric disorders largely remains stagnated. Drug repurposing offers a more efficient pathway compared with de novo drug discovery with lower cost and less risk. Various computational approaches have been applied to mine the vast amount of biomedical data generated over recent decades.

Integrative Analyses of Transcriptomes to Explore Common Molecular Effects of Antipsychotic Drugs.

There is little understanding of the underlying molecular mechanism(s) involved in the clinical efficacy of antipsychotics for schizophrenia. This study integrated schizophrenia-associated transcriptional perturbations with antipsychotic-induced gene expression profiles to detect potentially relevant therapeutic targets shared by multiple antipsychotics. Human neuronal-like cells (NT2-N) were treated for 24 h with one of the following antipsychotic drugs: amisulpride, aripiprazole, clozapine, risperidone, or vehicle controls.

Synthesis, characterization of novel ZnO/CuO nanoparticles, and the applications in photocatalytic performance for rhodamine B dye degradation.

Photocatalytic deg radation of environmental pollutants is being up to date for the treatment of contaminated water. In the present study, ZnO/CuO nanomaterials were successfully fabricated by a simple sol-gel method and investigate the photo-degradation of rhodamine B (RhB). The synthesized ZnO/CuO nanoparticles were characterized by X-ray diffraction (XRD), energy-dispersive spectroscopy (EDS), scanning electron microscopy (SEM), transmission electron microscopy (TEM), UV-Vis diffuse reflectance spectroscopy (UV-Vis-DRS), thermal analysis (TGA), surface charge, and Fourier transform infrared spectroscopy (FTIR).

Biological Mechanism(s) Underpinning the Association between Antipsychotic Drugs and Weight Gain.

Weight gain and consequent metabolic alterations are common side-effects of many antipsychotic drugs. Interestingly, several studies have suggested that improvement in symptoms and adverse metabolic effects are correlated. We used next generation sequencing data from NT-2 (human neuronal) cells treated with aripiprazole, amisulpride, risperidone, quetiapine, clozapine, or vehicle control, and compared with the Pillinger P-score (ranked from 0 to 1, indicating greater increase in weight gain and related metabolic parameters) to identify the genes most associated with the drugs' propensity to cause weight gain.

Transcriptional Modulation of the Hippo Signaling Pathway by Drugs Used to Treat Bipolar Disorder and Schizophrenia.

Recent reports suggest a link between positive regulation of the Hippo pathway with bipolar disorder (BD), and the Hippo pathway is known to interact with multiple other signaling pathways previously associated with BD and other psychiatric disorders. In this study, neuronal-like NT2 cells were treated with amisulpride (10 µM), aripiprazole (0.1 µM), clozapine (10 µM), lamotrigine (50 µM), lithium (2.

Construction of a G-quadruplex-specific DNA endonuclease.

We generated a novel G-quadruplex (G4)-specific endonuclease by fusing a G4 recognition domain of the RHAU helicase with a cleavage domain of the Fok1 nuclease. The fusion protein can specifically bind a parallel G4 and cleave a double-stranded DNA (dsDNA) next to it. The new endonuclease could be used to detect a G4 in a long dsDNA, providing a useful tool for mapping the formation of G4s in the genome.

[Collection of epidemiological data on oral and dental health in young children. Pilot study conducted with 212 children in 11 ONE centers in the Province of Liège].

Dental caries still affect 60 to 90 % of children attending school worldwide. We collected epidemiological data from 212 children under the age of 6 supervised by the Office of Birth and Childhood in the Province of Liège. We analyzed the relationships between the prevalence of dental caries in the studied population sample and the risk factors that are usually associated to dental carries.