Features of the content of matrix metalloproteinases ММР-9 and ММР-2 in mixed saliva of young individuals with dental caries against the background of different level of 25(OH) vitamin D in the body.

The content ofММР-9 and ММР-2 in oral fluid of 105 individuals between the ages of 19 and 23 has been researched.Of these, 42 people are individuals with dental caries and normal level of the active form of vitamin Din serum (25(OH)D >30ng/mL) and 42 people - with 25(OH)D <30 ng/mL level.The control group was composed of 21 individuals with low DMFt index (1,5) and a normal level of 25(OH)D in blood.

[Clinical and some biochemical features of rhinosinusitis of different etiology].

Identified pathogens, and studied the features of the clinical picture, the level of antioxidant activity, the number of TBA-active products, cytokines, chaperones proteins and antibodies against them in the serum of patients with fungal, viral, bacterial rhinosinusitis (RS) were studied. Most RS of viral nature are caused by rhinoviruses, in turn, the causative agent of bacterial RS, most often acts as a polyflora (S. epidermidis, S.

[Diagnostic and prognostic significance of urinary microvesicles].

In recent years, researchers have become increasingly interested in urinary microvesicles. They contain disease-specific biomarkers and can be used for predicting and diagnosing various diseases of the urinary system without resorting to invasive procedures. This review presents data on domestic and international research investigating the importance and potential application of urinary microvesicles for the diagnosis and prediction of diseases of the urinary system.

Impact of Fitness Status on the Optically Measured Hemodynamic Indexes.

The physiological characteristics of skin blood flow can be described in terms of the hemodynamic indices (HI). The HI is derived from the laser speckle characteristics, which are governed by the cutaneous blood flow. A miniaturized dynamic light-scattering sensor was used to measure the speckle pattern from the finger root.

Endothelial dysfunction in experimental hyperhomocysteinemia.

Unlabelled: To investigate the consequences of the endothelium dysfunction caused by the experimental hyperhomocysteinemia.

Methods: The experiment included the similar aged 42 white non-pedigree male rats with average weight of 150 grams. The rats were divided in two groups each of these having 21 species.

Heat shock protein 70 and anti-heat shock protein 70 antibodies in nasal secretions of patients with chronic rhinosinusitis.

Background: The issue of heat shock protein (HSP) 70 and anti-HSP70 antibodies in chronic rhinosinusitis (CRS) has never been explored.

Objective: To determine the nasal secretion (NS) levels of HSP70 and anti-HSP70 antibodies in patients with CRS with nasal polyps (CRSwNP) and patients with CRS without nasal polyps (CRSsNP), and to evaluate their associations with CRS clinical severity and correlation with NS interleukin (IL), IL-5 and interferon λ.

Methods: CRS severity was determined by Lund-Mackay scores.

Neuron-specific enolase in nasal secretions as a novel biomarker of olfactory dysfunction in chronic rhinosinusitis.

Background: Olfactory dysfunction is a diagnostic criterion for chronic rhinosinusitis (CRS). During chronic inflammation and olfactory neuronal damage in CRS, it is likely that neuron-specific enolase (NSE) can leak into nasal secretions (NS) and serum. Therefore, we postulated that NSE levels in NS and in circulation may be indicative of olfactory dysfunction in CRS.

Biomarker assessment in chronic rhinitis and chronic rhinosinusitis: Endothelin-1, TARC/CCL17, neopterin, and α-defensins.

Background: Chronic rhinitis (CR) is characteristically divided into several major clinical phenotypes: allergic rhinitis (AR); nonallergic, noninfectious rhinopathy (NAR); and chronic rhinosinusitis (CRS). CRS has two phenotypic variants: CRS with nasal polyps (CRSwNP) and CRS without nasal polyps (CRSsNP). An area of growing interest is to identify biologic markers that could assess different aspects of CR phenotypes.

Anticytokine autoantibodies in chronic rhinosinusitis.

Background: Anticytokine autoantibodies (AAbs) involve a great panel of cytokines both in healthy subjects and in patients with various diseases, but their incidence and pathophysiologic role are widely debated. The issue of AAbs in chronic rhinosinusitis (CRS) has never been explored.

Objective: The aim of the study was to check AAbs in patients with CRS and with nasal polyps (CRSwNP) and patients with CRS and without nasal polyps (CRSsNP).

Expression of TLR2 and TLR4 on peripheral blood monocytes during exacerbation of atopic dermatitis.

Background: In atopic dermatitis (AD), monocytes, which accumulate in the inflamed skin, are characterized by a significantly impaired Toll-like receptors (TLR) expression and TLR2-mediated cytokine secretion. However, data on expression of TLR on monocytes of peripheral blood (PB) in AD are not available.

Objective: To investigate TLR2 and TLR4 expression on PB monocytes during AD exacerbation and to assess the relationships between TLR expressions with AD clinical severity and with serum interleukin (IL) 4, IL-10, and IL-17a levels.

Plasma α-defensins are elevated during exacerbation of atopic dermatitis.

Background: There is no currently no information regarding the role of α-defensins in the pathophysiology of atopic dermatitis (AD).

Aim: To investigate levels of plasma α-defensins at exacerbation and remission of AD, and to assess their association with clinical severity of AD.

Methods: Patients with AD during exacerbation were recruited from the Dermatology department at the Chita Medical Academy (Chita, Russian Federation).

Lymphocyte Subpopulations and Cytokine Levels in Experimental Hyperhomocysteinemia.

Moderate exogenous hyperhomocysteinemia is associated with an increase in lymphocyte count (mainly at the expense of cytotoxic T cells), a 2-fold drop of the immunoregulatory index, and increase in TNF-α, IFN-γ, and IL-17α concentrations in the serum and in mononuclear cells. Homocysteine concentration in the mononuclears remains unchanged.

Plasma endothelin-1 levels during exacerbation of atopic dermatitis.

Background: Endothelin (ET) -1 was found to participate in the pathogenesis of bronchial asthma. At present, there is no information regarding the role of ET-1 in the pathophysiology of atopic dermatitis (AD).

Objective: We assessed blood ET-1 levels during the exacerbation of AD and during the 4 months postexacerbation period, to assess the relationships between blood ET-1 levels and clinical severity of AD and pruritus.

[Enzymes of azurophilic neutrophil granules and matrix metalloproteinase-2 as markers of the developmental stages of experimental respiratory distress syndrome].

Acute Respiratory Distress Syndrome was reproduced in the non-linear male rats by the original method. The animals were injected lysate 45-55 thousand rat neutrophils in 0.2 mi 0.

[Lung tissue factor expression changes in relation to the stage of respiratory distress syndrome in the presence of pneumonia in A/H1N1 influenza].

Lung paraffin sections were studied in 35 patients who had died during the epidemic of A/H1N1 influenza, including 10, 16, and 9 persons diagnosed with exudative, proliferative, and fibrotic stages of acute respiratory distress syndrome (ARDS), respectively. Lung tissue factor (TF) expression was determined in the sections by immunohistochemistry. TF was found to express endothelium, neutrophils, macrophages, type II alveolocytes, and bronchial and metaplastic epithelia.

[Serum, oral and gingival fluid levels of heat shock protein-70, cytokines and their autoantibodies by periodontal disease].

The study of 25 patients with periodontitis showed that the level of heat shock protein-70 (HSP70) and IL-1Β, IL-8 was increased in serum, buccal and gingival fluids as compared with healthy individuals, as well as the serum level of IgG autoantibodies both to HSP70 and to cytokines. In oral and gingival fluids sIgA autoantibodies to the same antigens were found. It was suggested that these autoantibodies can eliminate HSP-70 pro-inflammatory cytokines, thus regulating their levels in the inflammation area.

Experimental reproduction of stages of the respiratory distress syndrome.

The authors present a method for experimental reproduction of stages of the acute respiratory distress syndrome development, including the initial stage - acute pulmonary injury. Concentrated neutrophil transmitters served as the factor triggering the development of the process. The adequacy of the model was confirmed by the clear-cut morphological stages of the process.

[Expression of matrix metalloproteinase-2 and tissue inhibitor type 2 in the lung in relation to the stage of experimental respiratory distress syndrome].

Acute respiratory distress syndrome (ARDS) was reproduced in rats by endotracheal administration of 45-55 thousand lysed rat neutrophils (RF patent). In each stage of the syndrome, immunohistochemistry was used to determine the expression of matrix metalloproteinase-2 (MMP-2) and tissue inhibitor type 2 (MIMP-2) by lung cells. It was found that there was a substantial increment of MMP-2 expression in the fibroblasts, macrophages, endothelium, and fibroblasts in the exudative stage of ARDS.

[The use of Timalin in the treatment of the acute lung abscess].

The study was aimed to research levels of main acute inflammation phase peptides, coagulative and fibrinolitic plasma activity on the background of traditional treatment and with addition of Timalin in patients with acute lung abscess. The study demonstrated that induction of bioregulative therapy leads to faster normalization of main indicators of SIRS and plasma fibrinolitic activity and eliminates hypercoagulation.

[Content of antibodies to heat shock proteins in uncomplicated and complicated course of acute appendicitis].

The article presents information on the content and dynamics of changes in heat shock protein (HSP-70) in plasma and serum of patients with acute appendicitis before and after treatment. It was shown that higher content was noted in the group of patients with a complicated course. The use of traditional methods resulted in the decreased level of HSP-70 but in the group of patients with a complicated course the concentrations of shaperons remains elevated.

[The levels of neuron specific enolase and autoantibodies to it in lacrimal and intraocular fluids in patients with open-angle glaucoma (preliminary report)].

The concentration of neuron specific enolase (NSE) and autoantibodies (aAB) to it were studied in lacrimal and intraocular fluids in 9 patients with open-angle glaucoma (OAG) and 17 patients without this condition using ELISA. NSE concentration in lacrimalfluid of patients with OAG was showed to be 22 times as high and aAB 2 times as high as in control group.

[The level of endothelin-1 in blood serum and nasal secretion of patients presenting with chronic rhinitis].

The level of endothelin-1 (ET-1) in blood serum and nasal secretion was simultaneously determine by the relevant immunoenzyme assay in 23 of patients presenting with chronic rhinitis and in12 healthy subjects. It was shown that both vasomotor and chronic hypertrophic rhinitides are characterized by a similar (two-fold) rise in the serum ET-1 concentration. The six-fold increase of the ET-1 level in nasal secretion in chronic hypertrophic rhinitis compared with the respective normal parameter exceeds that in the vasomotor form of this pathology.

[The role of heat shock proteins in immune reactions in old-aged patients with chronic generalized periodontitis].

Chronic generalized periodontitis in the old-aged patients is accompanied by high levels of the antibodies to HSP, sIgA, IgM, IgG hyperproduction; elevated concentrations of IL-8 and IL-1alpha and low content of IL-4 in saliva and blood. Local anti-inflammatory therapy of chronic periodontitis does not restore normal immunity status. Appearance of antibodies to HSP plays a role in immune reactions, which maintain chronic inflammation of periodontium.

[Some cytokines and cytokines autoantibodies content in the blood serum, oral and gingival fluid in odontogenic maxillofacial abscesses].

Cytokines level and cytokines antibodies in the blood serum, oral and gingival fluids was studied in 15 patients with odontogenic maxillofacial abscesses. Increased level of the inflammatory cytokines (IL1β, IL6, IL8) and decreased level of the anti-inflammatory cytokines (IL4, IL10) in oral and dentigingival fluids. Besides, increased content of autoantibodies of class sIgA to IL8 and decreased content of autoantibodies of class sIgA to IL10 was noted.

[The levels of endothelin, neuron-specific enolase, and their autoantibodies in the serum and tear fluid of patients with type 2 diabetes mellitus].

Enzyme immunoassay was used to measure the serum and tear fluid concentrations of endothelin, neuron-specific enolase (NSE), and their autoantibodies in 19 patients with nonproliferative diabetic retinopathy (NDR). The serum and tear fluid levels of endothelin were shown to increase in patients with NDR. At the same time, the content of endothelin-1 autoantibodies rose in the blood and remained in the normal range in the tear fluid.