[The changes of expression and Fc-gamma-receptor's polypeptide composition of fetal small intestine enterocytes in Bos primigenius taurus L].

The expression and polypeptide composition of Fc-gamma-receptors of enterocytes from Bos primigenius taurus L. intestine at 3-, 5-, 7-, 9- month of fetal development was investigated. The results of immunobloting show similar composition of Fc-gamma-receptors extracted from apical and basolateral membranes.

[Obtaining of isolated enterocytes from the small intestine of cattle fetuses under the control of biochemical indices of cell fractions].

The modification of chemical method for obtaining of isolated bovine fetal small intestine enterocytes is experimentally substantiated in the paper. The necessity of time reduction of incubation of small intestine in solution of chelating agents for obtaining the high-cleared fraction of absorptive cells with the low degree of their destruction is proved.

[Acid-base homeostasis of the newborn calf].

Homeostasis of acid-base condition in newborn calves is ensured with metabolic, respiration--excretion and buffer systems, which allows for fast adaptation of animals to new living condition.

[Regularities in forming colostral immunity in newborn calves].

Formation of acid-base homeostasis in newborn calves takes place simultaneously with developing metabolic respiration-excretion and buffer system resulting in fast adaptation of animals to new environmental conditions.

[Dynamics of blood proteins spectra in normal and diarrheic long horn cattle during early ontogenesis].

The contains of blood plasma protein fractions and immunoglobulines were studied in adult cattle and in new born calves (just new born and age of 1, 2, 5 and 10 days) by the 10% PAAG EP (0.1% SDS) and radial immunodiffusion methods. It is shown that protein bands of 140-160 kD and immunoglobulines (G1, G2, M) are absent at the moment of birth.

[The isolation of plasma membrane fractions from the enterocytes of the small intestine in animals of different ages].

The described methodical conditions of obtaining apical and basolateral membranes from the suspension of isolated small intestine epithelial cells of cattle, that are more effective, in comparison with mucous membrane scrapings by certain criteria (material output, purity level, presence of pollution, vesiculosity and orientation of vesicles). It is determined, that apical and basolateral membranes of newborn animals have their specific features as to the amount of protein, pollution, purification in comparison with the mature animals. For several biochemical features the fractions of apical and basolateral membranes of enterocytes of the small intestine in mature and newborn animals might be recommended for the investigations of different biochemical processes, including the norm and pathology of the digestive tract.