Monopaternal superfecundation of quintuplets after transfer of two embryos in an in vitro fertilization cycle.

Objective: To present the first genetically proven identity of quintuplets in an IVF treatment cycle after transferring only two embryos.

Design: Case report.

Setting: IVF unit and obstetrics department of university-affiliated general hospital.

Tyrphostins that suppress the growth of human papilloma virus 16-immortalized human keratinocytes.

Human papilloma virus 16 (HPV16) is considered to be the causative agent for cervical cancer, which ranks second to breast cancer in women's malignancies. In an attempt to develop drugs that inhibit the malignant transformation of HPV16-immortalized epithelial cells, we examined the effect of tyrphostins on such cells. We examined the effect of tyrphostins from four different families on the growth of HPV16-immortalized human keratinocytes (HF-1) cells.

Lupus miliaris disseminatus faciei--the DNA of Mycobacterium tuberculosis is not detectable in active lesions by polymerase chain reaction.

There has been a controversy as to the origin of lupus miliaris disseminatus faciei (LMDF). It was originally thought to be associated with tuberculosis, due to its histopathological similarity. Recently, this association has been doubted, although there remain reported cases of LMDF associated with Mycobacterium tuberculosis.

Inhibitors of epidermal growth factor receptor kinase and of cyclin-dependent kinase 2 activation induce growth arrest, differentiation, and apoptosis of human papilloma virus 16-immortalized human keratinocytes.

Human papilloma virus 16 (HPV 16) is associated with cervical cancer and is therefore considered a major health risk for women. Immortalization of keratinocytes induced by HPV infection is largely due to the binding of p53 and Rb by the the viral oncoproteins E6 and E7, respectively, and is driven to a large extent by a transforming growth factor alpha/amphiregulin epidermal growth factor receptor autocrine loop. In this study, we show that the growth of HPV 16-immortalized human keratinocytes can be blocked by a selective epidermal growth factor receptor kinase inhibitor, AG 1478, and by AG 555, a blocker of cyclin-dependent kinase 2 (Cdk2) activation.

Simultaneous detection of three common sexually transmitted agents by polymerase chain reaction.

Objective: Human papillomaviruses, herpes simplex viruses, and Chlamydia trachomatis are very common infections of the genital tract. The purpose of our study was to develop a polymerase chain reaction-based assay for the simultaneous detection of these organisms from a single genital swab.

Study Design: To prove the technical feasibility of a simultaneous polymerase chain reaction assay for these organisms, a mixture of deoxyribonucleic acids extracted from cells infected by these three agents was amplified in the same tube with three different sets of primers corresponding to specific regions of the human papillomavirus genome, the herpes simplex virus 1 and 2 genomes, and the Chlamydia trachomatis plasmid, respectively.

Differential cooperation of a carcinogen with human papillomavirus type 6 and 16 DNAs in in vitro oncogenic transformation.

In contrast to the strong association of human papillomavirus (HPV) type 16 with genital malignancies, HPV 6 has been found essentially in benign genital lesions. In these studies we show that HPV type 6 and 16 DNAs behave differently also in their ability to transform NIH 3T3 cells in cooperation with the carcinogen N-methyl-N'-nitro-N-nitrosoguanidine (MNNG). Although we could show that both HPV-6- and HPV-16-transfected genomes were integrated and expressed in NIH 3T3 cells, only the NIH 3T3 cells which contained the HPV 16 genome became fully transformed after MNNG treatment, as assessed by their ability to form colonies in soft agar and to induce tumors in nude mice.

Oestrogen stimulates differential transcription of human papillomavirus type 16 in SiHa cervical carcinoma cells.

Human papillomavirus (HPV) type 16 is highly associated with cervical cancer, but it seems that cofactors such as hormones affect its potential oncogenicity. We have analysed the HPV-16 gene expression in response to sex hormones and glucocorticoids in SiHa cells, a human cervical carcinoma cell line. An eightfold induction of HPV-16 transcripts was obtained in oestrogen-treated SiHa cells.

Papillomaviruses in lesions of the lower genital tract in Israeli patients.

Human papillomaviruses (HPVs) have been strongly associated with benign lesions of the genital tract (condylomata) and with genital cancer of the vulva and cervix. Since the incidence of these lesions in Israel is considered to be low, we have studied the presence of HPV 6, 11, 16 and 18 DNAs in benign, premalignant and malignant tissue samples or gynecological swabs of the lower genital tract. HPV sequences were detected in 48 out of 66 condylomatous lesions (72%), 5/11 grades I-II intraepithelial neoplasia (45%), 4/6 grade III intraepithelial neoplasia (carcinoma in situ) (66.

Sector retinitis pigmentosa: a fluorescein angiographic study.

Flourescein angiography has been proved to be of value in the study of the chorioretinal abnormaliteis in sector retinitis pigmentosa. The findings from two patients, one being in an early and the other in a more advanced stage were analyzed. The pigment epithelium was found to be disturbed in a larger area than visible by ophthalmoscopy.