Iron-specific Signal Separation from within Heavy Metal Stained Biological Samples Using X-Ray Microtomography with Polychromatic Source and Energy-Integrating Detectors.

Biological samples are frequently stained with heavy metals in preparation for examining the macro, micro and ultra-structure using X-ray microtomography and electron microscopy. A single X-ray microtomography scan reveals detailed 3D structure based on staining density, yet it lacks both material composition and functional information. Using a commercially available polychromatic X-ray source, energy integrating detectors and a two-scan configuration labelled by their energy- "High" and "Low", we demonstrate how a specific element, here shown with iron, can be detected from a mixture with other heavy metals.

Electron tomography simulator with realistic 3D phantom for evaluation of acquisition, alignment and reconstruction methods.

Because of the significance of electron microscope tomography in the investigation of biological structure at nanometer scales, ongoing improvement efforts have been continuous over recent years. This is particularly true in the case of software developments. Nevertheless, verification of improvements delivered by new algorithms and software remains difficult.

Nonlinear immunofluorescent assay for androgenic hormones based on resonant structures.

We report for the first time the use of two photon fluorescence as detection method of affinity binding reactions. We use a resonant grating waveguide structure as platform enhancement for detecting the interaction between fluorescent labeled Boldenone, a non-natural androgenic hormone, and a specific anti-anabolic antibody. We were able to detect a surface coverage of approximately 0.

Role of photonic bandgaps in polarization-independent grating waveguide structures.

Polarization independence in a one-dimensional resonant grating waveguide structure involves the simultaneous excitation of two guided modes propagating in different directions. Possible simultaneous excitations occur when the two excited guided modes have either the same polarization, i.e.

Light modulation with electro-optic polymer-based resonant grating waveguide structures.

A novel modulator design incorporating an E-O polymer into a resonant grating waveguide structure is presented. Using purely polymeric material we developed a resonant grating waveguide structure having low loss and high finesse, with approximately 2nm spectral line width at 1.55 mum.