Differential modulation of mutant CALR and JAK2 V617F-driven oncogenesis by HLA genotype in myeloproliferative neoplasms.

It has been demonstrated previously that human leukocyte antigen class I () and class II () alleles may modulate JAK2 V617F and CALR mutation (CALRmut)-associated oncogenesis in myeloproliferative neoplasms (MPNs). However, the role of immunogenetic factors in MPNs remains underexplored. We aimed to investigate the potential involvement of genes in CALRmut+ MPNs.

Exploration of the role of NKG2D ligands MICA and MICB in JAK2 V617F-positive myeloproliferative neoplasms.

JAK2 V617F-driven myeloproliferative neoplasms (MPNs) can escape immune surveillance through PD-L1 up-regulation and HLA class I pathway down-regulation. To complement these data we assessed the role of major histocompatibility complex class I-related genes (MICA and MICB) in JAK2 V617F+ MPNs. Using high resolution genotyping we identified two protective alleles, MICA*008:01 and MICA*016.

The relevance of HLA class II genes in JAK2 V617F-positive myeloproliferative neoplasms.

In the present study we analyzed the relevance of HLA class II in JAK2 V617F-positive (JAK2 V617F+) myeloproliferative neoplasms (MPNs) focusing on genotype diversity, associations with specific alleles and haplotypes and the level of gene expression. One hundred and thirty-nine JAK2 V617F+ MPN patients and 1083 healthy controls, typed by Next generation sequencing (NGS) were included in the study. Multivariate generalized linear models with age as a covariate were applied for analysis of HLA-II allele and haplotype associations.

Presbyopia correction with a new Extended Depth of Focus Intraocular Lens.

Extended depth of focus intraocular lenses (EDoF IOLs) offer an expanded number of modalities for simultaneous cataract and presbyopia treatment. The objective of the current study was to assess clinical outcomes with a new mono-EDoF intraocular lens and to analyze the effect of different parameters on postoperative results. The inclusion criteria were defined as uneventful cataract surgery, no history of concomitant ocular disease, implantation of ZOE Primus-HD lens.

Probable HLA-mediated immunoediting of JAK2 V617F-driven oncogenesis.

Human leukocyte antigen class I (HLA-I) genotype has been found to influence cancer development through the presentation of mutational neoepitopes. However, our understanding of its effect on the development of myeloproliferative neoplasms (MPNs) remains limited. We aimed to elucidate the putative protective role of HLA-I alleles in the development of JAK2 V617F-driven MPNs using a population genetics approach.

Biclonal myeloma in renal failure.

Multiple myeloma is a clonal proliferation of the plasma cell line that accounts for approximately 10% of all hematological malignancies. It is characterized by abnormal growth of plasma cells producing monoclonal immunoglobulin or light chain (paraprotein), with subsequent development of osteolytic bone lesions, anemia, hypercalcemia, and renal failure. In 3-6% of myeloma patients, more than one monoclonal protein (usually two) is discovered, with different heavy or light chain or both.

Characterization of mesenchymal stem cells isolated from the human umbilical cord.

Numerous papers have reported that mesenchymal stem cells (MSCs) can be isolated from various sources such as bone marrow, adipose tissue and others. Nonetheless it is an open question whether MSCs isolated from different sources represent a single cell lineage or if cells residing in different organs are separate members of a family of MSCs. Subendothelial tissue of the umbilical cord vein has been shown to be a promising source of MSCs.

[Predicting in the course of scoliosis-related disorders of vertebral segmentation in children].

The article deals with the analysis of the natural course of +seoliosogenous disorders of vertebral segmentation in 26 patients during the first three years of life. The authors determined the prognostic criteria of the rate of increase of the deformity (angle of scoliosis) during the first visit to the doctor and the asymmetry index according to which the course of the disease can be prognosticated with a high degree of probability during the patient's first visit to the doctor. If the patient had radiographs taken at an interval of 6-12 months an additional test was applied-the apical vertebra growth coefficient.

[Morphological changes in the skin and blood in Sézary's syndrome].

The results of clinical and morphological analysis of two observations of the erythrodermic form of mycosis fungoides and Sezary's syndrome confirmed the opinion that Sezary's syndrome was a leukemic variant of mycosis fungoides. Morphological differences between atypical lymphocytes (Sezary's cells) and activated forms of lymphocytes are emphasized.

[Mononuclear epithelial infiltration in squamous cell skin cancer, allergic contact dermatitis and skin allograft].

Histological and electron microscopic examinations of cellular infiltration were carried out in squamous cell carcinoma of the skin in humans, in the zone of dinitrochlorobenzene allergic contact dermatitis in guinea pigs, and in allotransplant and the surrounding skin in mice. In all the processes studied, the epithelial part of the cell infiltration area was found to differ from the connective tissue area by cell composition. The epithelial sheets were shown to be infiltrated mostly with heterogenous lymphocytes as well as occasional macrophages forming simple and slit-like contacts with epithelial cells.

[Characteristics of the development of experimental allergic contact dermatitis].

Histological and electron microscopic examination of changes in the skin and blood revealed three different stages in the development of experimental allergic contact dermatitis (ACD). The primary contact reaction (24 hours) had features of nonspecific inflammation with some morphological signs of initial sensitization. Inflammation in the flare-up reaction (5-7 days) is the result of the immune process, and basophilic infiltration of the skin is the obligatory component of this reaction.