Fast-Track Development and Multi-Institutional Clinical Validation of an Artificial Intelligence Algorithm for Detection of Lymph Node Metastasis in Colorectal Cancer.

Lymph node metastasis (LNM) detection can be automated using artificial intelligence (AI)-based diagnostic tools. Only limited studies have addressed this task for colorectal cancer (CRC). This study aimed to develop of a clinical-grade digital pathology tool for LNM detection in CRC using the original fast-track framework.

Artificial Intelligence-Based Tool for Tumor Detection and Quantitative Tissue Analysis in Colorectal Specimens.

Digital pathology adoption allows for applying computational algorithms to routine pathology tasks. Our study aimed to develop a clinical-grade artificial intelligence (AI) tool for precise multiclass tissue segmentation in colorectal specimens (resections and biopsies) and clinically validate the tool for tumor detection in biopsy specimens. The training data set included 241 precisely manually annotated whole-slide images (WSIs) from multiple institutions.

Addition of seminal plasma proteins effecting the kinetic properties of canine spermatozoa.

The objective of this study is to evaluate the changes in the motility and kinetic patterns of canine spermatozoa, capacitated and decapacitated, after the addition of seminal plasma protein fractions with different molecular weight. It has been proposed that proteins in seminal plasma support the survival of the spermatozoa and exert a dual effect: capacitation and decapacitation. The seminal plasma from fresh ejaculates was subjected to chromatographic separation and four protein fractions were obtained.

Pathogenicity of Clinical OXA-48 Isolates and Impact of the OXA-48 IncL Plasmid on Virulence and Bacterial Fitness.

OXA-48 is the most common carbapenemase in Enterobacterales in Germany and one of the most frequent carbapenemases worldwide. Several reports have associated with a virulent host phenotype. To challenge this hypothesis, 35 OXA-48-producing clinical isolates of ( = 15) and ( = 20) were studied , employing the infection model and by whole-genome sequencing.

Chromatography analysis of seminal plasma proteins in buffalo semen samples with high and low cryotolerance.

The aim of this study was to analyse and identify specific buffalo seminal plasma proteins (SPPs) responsible for sperm cryotolerance during low temperature storage. Computer Assisted Sperm Analysis (CASA) of the motility and viability of buffalo spermatozoa was performed before freezing and after thawing. Two sample groups were formed - ejaculates with high cryotol- erance (group A) and low cryotolerance (group B).

Dataset illustrating the construction of dislocations and running dislocation dynamics using the periodic array of dislocation quadrupoles in FCC Al.

The data illustrates the process of creation of dislocation quadrupoles in FCC Al. The density of dislocations in our data is 3.8 × 10 m which is the lowest published to date and is comparable to the density of dislocations in real crystals.

[About stepwise uterine devascularization in early post-partum haemorrhages].

Bleedings are the leading cause for mother's mortality rate worldwide and that is why the authors face once more the abdominal operative methods for definite copying the early post partum haemorrhages (EPH). They share their experience gained already with 46 cases when EPH has definitively been copied by means of stepwise uterine devascularization of the vessels with atonic bleedings and ligation of a. iliaca interna presenting with laceration of the lower uterine segment and vagina as well.

Transposition of Saccharomyces cerevisiae Ty1 retrotransposon is activated by improper cryopreservation.

Ty1 is a retrotransposon of the yeast Saccharomyces cerevisiae whose transposition at new locations in the host genome is activated by stress conditions, such as exposure to UV light, X-rays, nitrogen starvation. In this communication, we supply evidence that cooling for 2 h at +4 degrees C followed by freezing for 1 h at -10 degrees C and 16 h at -20 degrees C also increased Ty1 transposition. The mobility of Ty1 was induced by cooling at slow rates (3 degrees C/min) and the accumulation of trehalose inside cells or the cooling at high rates (100 degrees C/min) inhibited significantly the induction of the transposition.

[Premature rupture of fetal membranes--diagnosis and management].

Purpose Of The Study: To investigate the reasons for preterm premature rupture of amnionic membranes (PPRAM), the methods for diagnosis and for identification of the syndrome of amnionic infection as well as to define the behaviour in relation to gestational age.

Material And Methods: The study covered 859 premature newborns of which, 283 (32.95%) were delivered by mothers presenting with PPRAM.

Mutagenic effect of freezing on mitochondrial DNA of Saccharomyces cerevisiae.

Although suggested in some studies, the mutagenic effect of freezing has not been proved by induction and isolation of mutants. Using a well-defined genetic model, we supply in this communication evidence for the mutagenic effect of freezing on mitochondrial DNA (mtDNA) of the yeast Saccharomyces cerevisiae. The cooling for 2 h at +4 degrees C, followed by freezing for 1 h at -10 degrees C and 16 h at -20 degrees C resulted in induction of respiratory mutations.

[COX-2 expression and ovarian cancer].

The aim of the present review is to study the significance of the COX-2 expression for unfavourable prognosis and aggressive clinicopathological parameters in certain malignancies. The main directions for future research on COX-2 as a prognostic factor of decreased survival and predictive factor of chemotherapy resistance in ovarian cancer are discussed.

[Intranatal asphyxia, prematurity and congenital infection--their role for brain damage in very low birth weight newborns].

The authors examined 273 newborns weighing less than 1500 g. Their birth weight was very low (1001-1500 g) in 179 but extremely low (< 1000 g) in 94 newborns. Neurological damage was proved in 101 cases (36.

[Stepwise uterine devascularization in postpartum hemorrhages].

The authors reported the results from a retrospective investigation of 10121 deliveries for a three-year period (1981-1983) as well as from a prospective study of 6239 child-births during a three-year period after a 20-year-long time lag, i.e., in 2000-2002.

Transgenic tobacco plants accumulating osmolytes show reduced oxidative damage under freezing stress.

We studied the reaction to the oxidative component of freezing in several tobacco lines, transformed with genes coding for enzymes involved in the synthesis of osmoprotectants (proline, fructan or glycine betaine) along with their wild type. The levels of some oxidative stress markers (leakage of electrolytes, hydrogen peroxide and malondialdehyde) as well as the activity of antioxidative enzymes catalase (EC 1.11.

[Eikenella corrodens in the etiology of tubo-ovarian abscesses in patients with intrauterine devices].

The authors analyzed the microbiological findings in 39 female patients with tubo-ovarian abscesses and intrauterine devices (IUD). An anaerobic microflora was isolated in 66.67% of the aspirates after puncture of cavum Douglasi (CD) as well as in 41.

[Pros and cons of magnesium sulfate as a tocolytic].

Magnesium sulphate (MgSO4) is with a proved effectiveness in cases of praeclampsia/eclampsia, but its use as a tocolytic is discussed in the last few years. That is why we had for an object to study its effect as a tocolytic in cases of abortions and premature labours. The study is prospective and if is made in I-st obstetric clinic of High Medical School--Pleven.

Investigation of Lactobacilli Strains during Lyophilization.

The Freezing Points of three lactobacilli strains cultivated in skim milk and MRS-broth were determined. Their survival was analyzed after lyophilization. A maximal preservation of the cell number of the R1 strain was found.

Freeze drying of biomaterials for the medical practice.

Experimentally determined thermophysical characteristics of the new antifibrinolytic bacteriostatic biomedical preparation CAPROCOL are reported. Experimental and theoretical temperature histories at selected locations in a sample of CAPROCOL undergoing freeze drying are compared. The advance of the moving phase interface is also predicted.