Identification of novel C-glycosylflavones and their contribution to flower colour of the Dutch iris cultivars.

Seventeen C-glycosylflavones including four novel ones, were isolated from the flowers of Dutch iris (Iris hollandica Hort. ex Todd.) cultivar 'Blue Diamond'.

Molecular cloning and characterization of O-methyltransferases from the flower buds of Iris hollandica.

In plants, O-methyltransferases (OMTs) play an important role in methylation of secondary metabolites, especially flavonoids and other phenylpropanoids, and two cDNA clones, IhOMT1 and IhOMT2 (Iris hollandica OMT), encoding OMTs were successfully isolated from a cDNA library of flower buds of I. hollandica. IhOMT1 encodes an open reading frame (ORF) of 365 amino acids with calculated molecular mass of 40,193Da and isoelectric point (pI) of 5.