Occurrence of cerebrocortical necrosis in a goat in Okinawa prefecture, Japan.

A 6-month-old male Nubian goat suddenly showed dullness, tachypnea, recumbency and opisthotonus in August 2011 in Okinawa, Japan. The goat was consequently necropsied: gross lesions in the brain included slightly swollen foci of yellowish discoloration on the cerebral hemisphere. Histopathologically, necrosis of the cortex with ischemic changes of neuronal cells was present, and swelling of the vascular endothelium, thickening of the basement membrane and diffuse infiltration of macrophages were observed in the cerebral lesion.

High-performance liquid chromatographic determination of ustiloxin A in forage rice silage.

We describe a high-performance liquid chromatographic method for determining ustiloxin A, a mycotoxin produced by Ustilaginoidea virens, in forage rice silage. Lyophilized silage samples were ground and extracted with water. The extracts were purified by solid-phase extraction and subjected to high-performance liquid chromatography using an octadecylsilane-bonded column.