Evaluation of a method for measuring tissue non-specific alkaline phosphatase activity in healthy subjects.

Background: Intestinal alkaline phosphatase (IAP) isozymes in the healthy human serum samples appears in two isoforms: normal-molecular-weight IAP (NIAP) and high-molecular-weight IAP (HIAP). We have demonstrated that the reference range for serum alkaline phosphatase (ALP) activity is higher in blood group B and O antigen secretors than in the tested other blood groups, for the appearance of these isoforms is depended on blood group B or O antigen secretors.

Methods: We assessed a diethanolamine-L-phenylalanine (DEA-Phe) method for measuring tissue non-specific alkaline phosphatase (TNAP).

[Estimation of blood-group-dependent hyperphosphatasemia in healthy subjects by agarose gel electrophoresis].

Intestinal alkaline phosphatase (IAP) isozymes in the healthy human sera appears in two isoforms: normal-molecular-weight IAP (NIAP) and high-molecular-weight IAP (HIAP). We have demonstrated that the reference range for ALP activity is higher in blood group B and O secretors than in the other blood groups, for the appearance of these isoforms is depended on blood group B or O secretors. We currently reported that blood groups dependent hyperphosphatasemia in healthy subjects only appeared in blood group B or O secretors.

[Incidence and characteristics of intestinal hyperphosphatasemia in healthy subjects].

We recently reported that two intestinal alkaline phosphatase (IAP) isoforms, high molecular mass IAP (HIAP) and normal molecular mass IAP (NIAP), appear in healthy serum with our Triton-PAGE method for determination of ALP isozymes. In addition, HIAP is chiefly present in blood group B or O secretors, and a large amount of NIAP is secreted into the circulation in blood group B or O secretors. In the present paper, we examined incidences and characteristics of intestinal hyperphosphatasemia in healthy subjects and blood groups dependent hyperphosphatasemia.

The effect of different buffers and amounts of intestinal alkaline phosphatase isoforms on total alkaline phosphatase activity.

Background: The transphosphorylating accepter buffers (2-amino-2-methyl-1-propanol, AMP; N-methyl-D-glucamine, MEG; diethanolamine, DEA and 2-ethylaminoethanol, EAE) have been widely used for the measurement of serum total alkaline phosphatase activity (ALP) in clinical laboratories, and the individual isozyme are activated differently by respective buffers.

Materials And Methods: We examined the activity of serum ALP using four buffers with levels of both high molecular weight intestinal alkaline phosphatase (HIAP) and normal molecular weight intestinal alkaline phosphatase (NIAP). We classified 80 healthy subjects into two groups of blood group B or O secretors (n=36) and other blood groups (n=44).