Temperature-gradient dependent detection of target DNA oligomers using DNA-immobilized open tubular capillary column.

A DNA-immobilized open tubular capillary column (DNA-immobilized OTC) system was developed for analysis and separation of target DNA oligomers. By combining the DNA-immobilized OTC column with a nano/micro-flow pump and a high-sensitivity UV detector, we succeeded in small-scale selective detection of target DNA oligomers (> 0.02 pmol).