Publications by authors named "Tsute Chen"

The oral cavity may play a role as a reservoir and in the transmission and colonization of Helicobacter pylori. The route of transmission for H. pylori is not fully understood.

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Background: , a pathobiont in periodontal disease, contributes to alveolar bone destruction. We assessed the efficacy of a new targeted antimicrobial, FP-100, in eradicating from the oral microbial community and and evaluated its effectiveness in reducing bone loss in a mouse periodontitis model.

Methods: A multispecies bacterial community was cultured and treated with two concentrations of FP-100 over two days.

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Article Synopsis
  • - Periodontitis is linked to changes in oral microbiomes, specifically affecting the tongue and saliva, and this study investigates these dysbiosis effects using a specific dysbiosis index.
  • - The research analyzed data from four studies comprising 328 microbiome samples, revealing significant increases in microbial dysbiosis in saliva and subgingival biofilm among periodontitis patients, with a reliable diagnostic accuracy for periodontitis.
  • - Although the impact on tongue microbiomes needs more research, the study found that salivary dysbiosis could serve as a valuable biomarker for periodontal disease assessment.
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Background: Subgingival dental plaque is an ecosystem playing a key role in supporting both oral health and systemic health. Menopause-related changes have the potential to disrupt its balance, which is crucial to postmenopausal well-being. Our study explored how circulating estradiol levels correlate with subgingival microbial composition using checkerboard DNA-DNA hybridization in premenopausal and postmenopausal women.

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Restorative dental materials can frequently extend below the gingival margin, serving as a potential haven for microbial colonization, and altering the local oral microbiome to ignite infection. However, the contribution of dental materials on driving changes of the composition of the subgingival microbiome is under-investigated. This study evaluated the microbiome-modulating properties of three biomaterials, namely resin dental composites (COM), antimicrobial piezoelectric composites (BTO), and hydroxyapatite (HA), using an optimized subgingival microbiome model derived from patients with periodontal disease.

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Article Synopsis
  • Children with severe early childhood caries (S-ECC) often require extensive treatment, and this study aims to assess how such treatments affect their oral microbiome in the short term.
  • The research involved 33 children aged 2-4 who underwent comprehensive caries treatment, including restorations and extractions, with plaque samples taken before and one month after the procedure for analysis.
  • Findings revealed that while overall microbial diversity increased post-treatment, the complexity of interbacterial interactions decreased, potentially indicating a healthier microbiome after caries therapy.
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Background: Grade C (previously aggressive) periodontitis (GCP) in adolescents is prevalent in certain parts of Africa where it is associated with JP2 genotype, a highly virulent strain of . The aim of this study was to characterize the subgingival bacteriome in Moroccan subjects with GCP positive to JP2 genotype.

Methods: Subgingival plaque samples were collected from shallow and deep pockets of 8 subjects with GCP (17.

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is a ubiquitous commensal species of the oral cavity commonly involved as an opportunistic pathogen in cardiovascular infections. In this study, we investigated the functions of endopeptidase O (PepO) and a C3-degrading protease (CppA) in the systemic virulence of . Isogenic mutants of and obtained in strain SK36 showed increased susceptibility to C3b deposition and to opsonophagocytosis by human polymorphonuclear neutrophils (PMN).

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Article Synopsis
  • Pg is an oral pathogen linked to periodontal disease, showing strain diversity influenced by factors like viral phage infection, which is largely overlooked in previous studies.
  • Research found that Porphyromonas gingivalis is infected by diverse prophages that can potentially alter its physiology and interactions, indicating a complex ecological dynamic.
  • The study highlights that phages and anti-phage defense mechanisms are significant contributors to the genetic variability within Pg, reshaping our understanding of its role in periodontal disease progression.
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Introduction: Infective endocarditis (IE) is an inflammatory disease usually caused by bacteria that enter the bloodstream and establish infections in the inner linings or valves of the heart, including blood vessels. Despite the availability of modern antimicrobial and surgical treatments, IE continues to cause substantial morbidity and mortality. Oral microbiota is considered one of the most significant risk factors for IE.

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Article Synopsis
  • The study explores the relationship between the microbiome and host transcriptomes in oral squamous cell carcinoma (OSCC) using advanced techniques beyond traditional 16S rRNA gene sequencing.
  • Analysis of tissue samples and healthy controls revealed a unique microbiome in OSCC that is low in abundance yet active, with specific bacteria and bacteriophages linked to cancer-related gene expression.
  • Findings suggest that interactions between the microbiome and host may contribute to oral cancer development, highlighting the need for further experimental validation of these mechanisms.
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Understanding the axis of the human microbiome and physiological homeostasis is an essential task in managing deep-space-travel-associated health risks. The NASA-led Rodent Research 5 mission enabled an ancillary investigation of the gut microbiome, varying exposure to microgravity (flight) relative to ground controls in the context of previously shown bone mineral density (BMD) loss that was observed in these flight groups. We demonstrate elevated abundance of Lactobacillus murinus and Dorea sp.

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Background: Current periodontal treatment involves instrumentation using hand and/or ultrasonic instruments, which are used either alone or in combination based on patient and clinician preference, with comparable clinical outcomes. This study sought to investigate early and later changes in the subgingival biofilm following periodontal treatment, to identify whether these changes were associated with treatment outcomes, and to investigate whether the biofilm responded differently to hand compared with ultrasonic instruments.

Methods: This was a secondary-outcome analysis of a randomized-controlled trial.

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The COVID-19 pandemic has resulted in the widespread use of N95 respirators and surgical masks, with anecdotal reports among healthcare providers and the public of xerostomia, halitosis, and gingivitis, a consortium of symptoms colloquially termed "mask mouth". However, this has not been scientifically verified. The aim of this study was to assess changes in salivary flow rate, gingival health status and oral microbiome associated with prolonged mask use.

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Modeling subgingival microbiome in health and disease is key to identifying the drivers of dysbiosis and to studying microbiome modulation. Here, we optimize growth conditions of our previously described subgingival microbiome model. Subgingival plaque samples from healthy and periodontitis subjects were used as inocula to grow normobiotic and dysbiotic microbiomes in MBEC assay plates.

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Article Synopsis
  • The study aimed to understand how oral and gut microbes could help differentiate between various periodontal conditions such as health, gingivitis, and periodontitis.
  • The research involved analyzing saliva and fecal samples from participants using 16S rRNA gene sequencing to assess microbial composition and identify correlations with periodontal health parameters.
  • Findings indicated some microbial differences correlated with periodontitis severity, including specific bacteria linked to inflammation, with a strong overall connection between oral and gut microbiomes highlighted in patient classifications.
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Early childhood caries (ECC) is not only the most common chronic childhood disease but also disproportionately affects underserved populations. Of those, children living in Thailand have been found to have high rates of ECC and severe ECC. Frequently, the cause of ECC is blamed on a handful of cariogenic organisms, such as and .

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Background And Objectives: The aim of this study was to examine the salivary microbiome in healthy peri-implant sites and those with peri-implantitis.

Methods: Saliva samples were collected from 21 participants with healthy peri-implant sites and 21 participants with peri-implantitis. The V4 hypervariable region of the 16S rRNA gene was sequenced using the Ion Torrent PGM System (Ion 318™ Chip v2 400).

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Background: This study was conducted to compare the microbiomes, the levels of lipopolysaccharides (LPS), lipoteichoic acid (LTA), and cytokines (interleukin [IL]-1β and tumor necrosis factor-alpha [TNF-α]), before and after chemomechanical preparation (CMP) of the root canals (RC) and their associated periodontal pockets (PP) in teeth with combined EPL.

Materials: Samples were taken from 10 RC and PP, before and after CMP. The microbiomes (next-generation sequencing, V3-V4 hypervariable region of the 16S rRNA gene), microbiome diversity (bioinformatics analyses), LPS (limulus amebocyte lysate), LTA, IL-1β, and TNF-α (ELISA) were evaluated.

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We report the complete genome of Arachnia rubra strain DSM 100122. The genome is 3.32 Mb, with a GC content of 64.

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In burn patients Pseudomonas aeruginosa infection is a major cause of morbidity. Analysis of the pathogen's gene expression as it transitions from colonization to acute and then biofilm wound infection may provide strategies for infection control. Toward this goal, we seeded log-phase P.

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Periodontal and Endodontic diseases are biofilm-related diseases. The presence of microorganisms in root canals (RCs) and the complex microbiota of periodontal pockets (PPs) contribute to the development of endodontic-periodontal diseases. This study performed a systemic analysis using state-of-the-art sequence data to assess the microbial composition of infected RCs and PPs to further assess the microbiota and verify the possibility of cross-infection between these sites.

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Periodontitis has been associated with many systemic diseases and conditions, including metabolic syndrome. Metabolic syndrome is a cluster of conditions that occur concomitantly and together they increase the risk of cardiovascular disease and double the risk of type 2 diabetes. In this review, we focus on the association between metabolic syndrome and periodontitis; however, we also include information on diabetes mellitus and cardiovascular disease, since these two conditions are significantly intertwined with metabolic syndrome.

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Background: The impact of glycemic fluctuation under diabetic condition on peri-implantitis in diabetic patients remains unclear. We hypothesized that glycemic fluctuation has greater adverse effect on experimental peri-implantitis, compared with sustained high blood glucose in diabetes.

Results: Maxillary left first and second molars of diabetic db/db mice were extracted and were replaced with one dental implant in the healed edentulous space.

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