2-Hydroxyl hispolon reverses high glucose-induced endothelial progenitor cell dysfunction through the PI3K/Akt/eNOS and AMPK/HO-1 pathways.

Background And Purpose: In diabetes (DM), elevated blood sugar levels contribute to the overproduction of reactive oxygen species (ROS), leading to endothelial progenitor cell (EPC) dysfunction. This study aimed to determine the potential of 2-hydroxy hispolon (2HH), a derivative of hispolon, to reverse high glucose-induced EPC dysfunction.

Experimental Approach: Under in vitro high-glucose (HG) conditions, we investigated the effects of 2HH on three types of angiogenic cells: outgrowth endothelial cells (OECs), circulating angiogenic cells (CACs) and endothelial cells (ECs).